中国中药杂志

2020, v.45(18) 4466-4471

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红景天苷在小鼠体内药代动力学及组织分布特征研究
Study on pharmacokinetics and tissue distribution characteristics of salidroside in mice

鲍小强;吴海楠;
BAO Xiao-qiang;WU Hai-nan;School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University;School of Forestry, Nanjing Forestry University;

摘要(Abstract):

建立了小鼠血浆和组织中红景天苷的含量测定方法,研究红景天苷在小鼠体内的药代动力学及组织中分布特征。以12.30,36.90,73.80 mg·kg~(-1) 3个剂量红景天苷灌胃小鼠,采集血浆和组织样品,然后通过UPLC-MS/MS检测各样品中红景天苷的浓度,研究红景天苷血浆药代动力学参数及组织分布情况。结果显示,在小鼠血浆和组织中,红景天苷在43.40~5 556 ng·mL~(-1)呈现良好的线性关系。日内、日间精密度均小于15%,提取回收率在79.50%~98.20%。研究发现红景天苷在血浆浓度较高,在0.5 h血药浓度达到最大值,在6 h后血浆中就检测不到红景天苷,说明红景天苷吸收快、清除也快。在组织中,肝和肾中浓度较高,说明红景天苷通过肝肾代谢和排泄。该实验建立的红景天苷检测方法操作简便、精密度良好,适用于红景天苷在小鼠体内的药代动力学和组织分布研究,为红景天苷作用机制研究及药物开发提供一定的参考。
The present study aims to establish a method for determination of salidroside in plasma and tissues of mice, and to study the pharmacokinetics and distribution characteristics of salidroside in mice. After intragastrically administered of salidroside at doses of 12.30, 36.90 and 73.80 mg·kg~(-1) to mice, plasma and tissue samples were collected, and the concentration of salidroside in each sample was measured by UPLC-MS/MS to study plasma drug parameters and tissue distribution of salidroside. Salidroside showed a good linear relationship in the plasma and tissues of mice in the concentration range of 43.40-5 556 ng·mL~(-1). The intraday and interday precision are less than 15%, and the accuracy is between 79.50% and 98.20%. The tissue distribution study found that salidroside had higher plasma concentration, and the plasma concentration reached the maximum at 0.5 h. After 6 h, no salidroside was detected in the plasma, indicating that salidroside has good solubility and absorption fast, clearance is also fast. In tissues, the concentration of liver and kidney tissues is higher, indicating that salidroside liver and kidney are the main metabolic and excretory organs. The established method is easy to operate and has good precision. It is suitable for salidroside in mice. Pharmacokinetics and tissue distribution studies provide a reference for the mechanism of action and drug development of salidroside.

关键词(KeyWords): 红景天苷;UPLC-MS/MS;药代动力学;组织分布
salidroside;UPLC-MS/MS;pharmacokinetics;tissue distribution

Abstract:

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基金项目(Foundation):

作者(Author): 鲍小强;吴海楠;
BAO Xiao-qiang;WU Hai-nan;School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University;School of Forestry, Nanjing Forestry University;

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DOI: 10.19540/j.cnki.cjcmm.202000623.202

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