彭颖;金晶;杨静玉;吴春福;李晓波;
PENG Ying1,2,JIN Jing1,WU Chun-fu2,YANG Jing-yu2,LI Xiao-bo1(1.School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200030,China;2.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

• 1:上海交通大学药学院
• 2:沈阳药科大学药学院

摘要(Abstract)：

目的:研究3种健脾补气方药对脾气虚证大鼠肠道菌群的影响,并探讨其作用机制。方法:利用运动疲劳兼饥饱失常法复制大鼠脾气虚证模型,采用ERIC-PCR(enterobacterial repetitive intergenic consensus-PCR)指纹图谱分析技术观察比较灌胃给予四君子汤(含生药10.8,3.6,1.2 g.kg-1)、理中汤(16.2,5.4,1.8 g.kg-1)和补中益气汤(14.4,4.8,1.6 g.kg-1)3种临床常用健脾补气方药对脾气虚证大鼠的治疗作用,研究其对肠道菌群的影响。结果:造模后大鼠肠道菌群ERIC-PCR指纹图谱条带位置、数量及丰度与健康状态相比均发生改变,说明其肠道菌群发生了一定变化。给予3种健脾补气方药后,大鼠ERIC-PCR指纹图谱多样性指数(shannon's index,H′)有所回升,各给药治疗组与健康时期的相似性系数(sorenson's pairwise similarity coefficient,Cs)与模型组相比显著升高。结论:ERIC-PCR指纹图谱分析技术是分析肠道菌群变化较理想的1种方法,该技术可用于脾气虚证本质和治疗脾气虚证药物的筛选或评价相关研究中。3种健脾补气方药对肠道菌群均具有一定的调节作用,其对肠道菌群的调节可能为其治疗脾气虚证的作用机制之一。
Objective:To determine the effect of three Jianpi Buqi recipes on intestinal microflora of Pi-qi-deficiency rat with molecular biology methods and attempt to clarify the mechanisms of therapy.Method:Rat model of Pi-qi-deficiency was induced by exercise fatigue simultaneously diet incontinence and then administrated with the decoctions of Si-jun-zi-tang(including crude drug)(10.8,3.6,1.2 g·kg-1),Li-zhong-tang(16.2,5.4,1.8 g·kg-1) and Buzhong Yiqi-tang(14.4,4.8,1.6 g·kg-1) recipes which were in common use of clinic of traditional Chinese medicine(TCM).With application of ERIC-PCR(enterobacterial repetitive intergenic consensus-PCR) fingerprint analysis,the efficacy on Pi-qi-deficiency and perturbation of intestinal mircoflora of these recipes were examined and evaluated.Result:Remarkable differences of ERIC-PCR profile were found between Pi-qi-deficiency state rats and normal rats.The Shannon's Index(H′) was increased when treated with all doses of three Jianpibuqi recipes decoctions and Sorenson′s pairwise similarity coefficient(Cs) was also increased significantly after treatment.Conclusion:ERIC-PCR fingerprint can be utilized as an initial screening of changes of the composition of bacterial communities that are associated with the development of intestinal disease.It can also be used to build a better understanding of Pi-qi-deficiency and to investigate the pharmacodynamic effect of TCM on Pi-qi-deficiency.Three Jianpi Buqi recipes are demonstrated to produce pronounced recovery activities on intestinal microflora of Pi-qi-deficient rats,which may be considered one of mechanisms of their performances.

关键词(KeyWords)： 脾气虚证;肠道菌群;ERIC-PCR;健脾补气方药
Pi-qi-deficiency;intestinal microflora;ERIC-PCR;Jianpi Buqi recipes

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金重大项目(90409018)

作者(Authors): 彭颖;金晶;杨静玉;吴春福;李晓波;
PENG Ying1,2,JIN Jing1,WU Chun-fu2,YANG Jing-yu2,LI Xiao-bo1(1.School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200030,China;2.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

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