倪梁红;赵志礼;吴靳荣;熊波;陆佳妮;嘎务;
NI Liang-hong;ZHAO Zhi-li;WU Jin-rong;XIONG Bo;LU Jia-ni;GAAWE Dorje;School of Pharmacy,Shanghai University of Traditional Chinese Medicine;Tibetan Traditional Medical College;

• 1:上海中医药大学中药学院
• 2:西藏藏医学院

摘要(Abstract)：

通过野外采集、分类学鉴定及多基因组多片段相结合,对2012年版《西藏自治区藏药材标准》中"桑蒂"、"莪德哇"和"叶兴巴"3种藏药基原植物的遗传多样性进行分析研究。经鉴定它们的基原植物分别为毛萼獐牙菜Swertia hispidicalyx、全萼秦艽Gentiana lhassica和齿叶玄参Scrophularia dentata;直接测定各样品的核糖体DNA ITS区、叶绿体mat K,rbc L,rpo C1,trn L(UAA),psb A-trn H,atp B-rbc L,trn S(GCU)-trn G(UCC),rpl20-rps12,trn L(UAA)-trn F(GAA)及线粒体nad1第2内含子序列共93条;毛萼獐牙菜、齿叶玄参的ITS区存在杂合,通过TA克隆法获得60条克隆序列。将克隆序列分为不同的基因型;基于所获全部序列及近缘物种相关序列,探讨3种藏药基原植物的遗传多样性,构建了全萼秦艽的DNA条形码。该工作可为不同遗传背景的藏药基原植物物种鉴定DNA条形码的建立提供新思路及基础资料。
The genetic diversity of three Tibetan herbs,i. e.,Sang-Di,E-Dewa and Ye-Xingba( Tibetan names),was studied based on the field collection,specimen identification and DNA sequence analysis. Swertia hispidicalyx,Gentiana lhassica and Scrophularia dentata,as the original plants of the three Tibetan herbs,were collected and identified. The regions of ITS,mat K,rbc L,rpo C1,trn L( UAA),psb A-trn H,atp B-rbc L,trn S( GCU)-trn G( UCC),rpl20-rps12,trn L( UAA)-trn F( GAA) and nad1 2ndintron were amplified and sequenced. The ITS regions of S. hispidicalyx and S. dentata were cloned and sequenced,and the sequences were classified into different genotypes. All the sequences were analyzed and compared with those of closely related species. Our studies may provide reference for the genetic diversity analysis and molecular identification of the three Tibetan herbs.

关键词(KeyWords)： 藏药;獐牙菜属;龙胆属;玄参属;克隆测序;遗传多样性
Tibetan herb;Swertia;Gentiana;Scrophularia;clone sequencing;genetic diversity

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81173654)

作者(Authors): 倪梁红;赵志礼;吴靳荣;熊波;陆佳妮;嘎务;
NI Liang-hong;ZHAO Zhi-li;WU Jin-rong;XIONG Bo;LU Jia-ni;GAAWE Dorje;School of Pharmacy,Shanghai University of Traditional Chinese Medicine;Tibetan Traditional Medical College;

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