倪梁红;赵志礼;吴靳荣;熊波;陆佳妮;嘎务;

• 1:上海中医药大学中药学院
• 2:西藏藏医学院

摘要(Abstract)：

通过野外采集、分类学鉴定及多基因组多片段相结合,对2012年版《西藏自治区藏药材标准》中"桑蒂"、"莪德哇"和"叶兴巴"3种藏药基原植物的遗传多样性进行分析研究。经鉴定它们的基原植物分别为毛萼獐牙菜Swertia hispidicalyx、全萼秦艽Gentiana lhassica和齿叶玄参Scrophularia dentata;直接测定各样品的核糖体DNA ITS区、叶绿体mat K,rbc L,rpo C1,trn L(UAA),psb A-trn H,atp B-rbc L,trn S(GCU)-trn G(UCC),rpl20-rps12,trn L(UAA)-trn F(GAA)及线粒体nad1第2内含子序列共93条;毛萼獐牙菜、齿叶玄参的ITS区存在杂合,通过TA克隆法获得60条克隆序列。将克隆序列分为不同的基因型;基于所获全部序列及近缘物种相关序列,探讨3种藏药基原植物的遗传多样性,构建了全萼秦艽的DNA条形码。该工作可为不同遗传背景的藏药基原植物物种鉴定DNA条形码的建立提供新思路及基础资料。

关键词(KeyWords)： 藏药;獐牙菜属;龙胆属;玄参属;克隆测序;遗传多样性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81173654)

作者(Author): 倪梁红;赵志礼;吴靳荣;熊波;陆佳妮;嘎务;

Email:

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