中国中药杂志

2021, v.46(16) 4034-4039

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UPLC-MS/MS测定铁皮石斛及其同属近源石斛品种中烟酰胺单核苷酸和烟酰胺腺嘌呤二核苷酸含量
Determination of β-nicotinamide mononucleotide and nicotinamide adenine dinucleotide in Dendrobium officinale and congeneric species by UPLC-MS/MS

刘晓谦;杨红;赵靖源;孟辰笑凝;李春;张东;陈两绵;闫钰;郭中原;王智民;易红;
LIU Xiao-qian;YANG Hong;ZHAO Jing-yuan;MENG Chen-xiao-ning;LI Chun;ZHANG Dong;CHEN Liang-mian;YAN Yu;GUO Zhong-yuan;WANG Zhi-min;YI Hong;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;Capital Medical University;

摘要(Abstract):

烟酰胺单核苷酸(β-nicotinamide mononucleotide, NMN)与烟酰胺腺嘌呤二核苷酸(nicotinamide adenine dinucleotide, NAD~+,辅酶I)是近五年来风靡全球的抗衰老成分,口服NMN后在体内转化为NAD~+而发挥其抗衰老之功效。为揭示《神农本草经》记载石斛"轻身延年"之功效,该文首次建立了利用超高效液相色谱串联质谱仪(UPLC-MS/MS)同步测定NMN与NAD~+含量测定方法,并测定了铁皮石斛及其同属近源石斛的NMN与NAD~+含量。经方法学验证,该方法的精密度、重复性、稳定性良好,NMN与NAD~+的平均回收率分别为77.58%与80.70%,RSD分别为3.6%与4.3%。34批样品测定结果表明,铁皮石斛的NMN含量高于其他同属近源石斛品种;鲜品铁皮石斛中NMN质量分数高达0.931 9μg·g~(-1),意外地发现,仅有铁皮石斛含有抗衰老功效成分NAD~+,且其含量更是高于NMN 3倍以上。这也许是铁皮石斛被祖先列为"九大仙草"之首的内在依据;同时发现不同的产地加工方法对石斛中NMN和NAD~+的含量影响较大,其含量高低表现为鲜品>烤条>枫斗,提示食用方法以鲜用为佳。
As anti-aging ingredients, β-nicotinamide mononucleotide(NMN) and nicotinamide adenine dinucleotide(NAD~+) have attracted worldwide attention in recent years. After oral administration, NMN can be converted into NAD~+ in vivo and the latter is the actual ingredient which exerts anti-aging effect. In order to explore the "rejuvenating and anti-aging" effect of Dendrobium officinale, which was firstly recorded in Shennong′s Herbal Classic of Materia Medica, this study established the quantitative method of UPLC-MS/MS for simultaneous determination of NMN and NAD~+ in D. officinale and the congeneric species for the first time, and 34 batches of samples were detected. UPLC conditions are as follows: ACQUITY UPLC HSS T3 column(2.1 mm × 100 mm, 1.8 μm), gradient elution with acetonitrile-0.1% formic acid in water at the flow rate of 0.3 mL·min~(-1), and column temperature of 40 ℃. MS conditions were scanned electrospray ionization source and multiple reaction monitoring mode. The method was verified by systematic methodology. The mean recoveries of NMN and NAD~+ were 77.58% and 80.70%, respectively, with RSD of 3.6% and 4.3%, separately. All results showed that the content of NMN was higher in D. officinale than in the other congeneric species. Particularly, the content in fresh D. officinale stems was as high as 0.931 9 μg·g~(-1). NAD~+ was only found in D. officinale and the content was three times higher than that of NMN. This may be the reason that D. officinale topped the "nine famous anti-aging herbs". In addition, processing method influences the content of NMN and NAD~+ in Dendrobium. Specifically, the content of NMN and NAD~+ was in the order of fresh Dendrobium stems > dried Dendrobium stem segments > spiral or spring-like dried Dendrobium stems.

关键词(KeyWords): 铁皮石斛;石斛;烟酰胺单核苷酸;烟酰胺腺嘌呤二核苷酸;UPLC-MS/MS
Dendrobium officinale;Dendrobium;NMN;NAD~+;UPLC-MS/MS

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2017YFC1701900)

作者(Author): 刘晓谦;杨红;赵靖源;孟辰笑凝;李春;张东;陈两绵;闫钰;郭中原;王智民;易红;
LIU Xiao-qian;YANG Hong;ZHAO Jing-yuan;MENG Chen-xiao-ning;LI Chun;ZHANG Dong;CHEN Liang-mian;YAN Yu;GUO Zhong-yuan;WANG Zhi-min;YI Hong;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;Capital Medical University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20210507.303

参考文献(References):

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