中国中药杂志

2013, v.38(02) 186-192

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

清火栀麦片中化学成分的SPE-HPLC-ESI-MS~n快速识别与鉴定
Rapid recognition and identification of chemical constituents in Qinghuo Zhimai tablets by SPE-HPLC-ESI-MS~n

张加余;乔延江;高晓燕;张倩;马群;卢建秋;
ZHANG Jia-yu1,2,QIAO Yan-jiang1*,GAO Xiao-yan2,ZHANG Qian1,MA Qun1,LU Jian-qiu2*(1.School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 100102,China; 2.Center of Scientific Experiment,Beijing University of Chinese Medicine,Beijing 100029,China)

摘要(Abstract):

目的:建立一种固相萃取-高效液相色谱-电喷雾串联质谱(SPE-HPLC-ESI-MSn)分析方法,对清火栀麦片中主要的化学成分进行快速识别与鉴定。方法:根据HPLC-ESI-MSn负离子模式下的分子离子峰以及碎片离子获得化合物的结构信息,并通过与文献数据或对照品对照,确定化合物可能的结构。结果:通过对清火栀麦片及其组方药味进行比较分析,归属并鉴定了其中的39个化学成分,包括7个咖啡酰奎尼酸、7个环烯醚萜、6个二萜内酯、5个高异黄酮、13个甾体皂苷以及1个黄酮类成分。结论:该研究为鉴别清火栀麦片中的化学成分提供了一种简便、快速的方法。
Objective: To establish a method for analyzing solid phase extraction-high performance liquid chromatography-electrospray ionization tandem mass spectrometry(SPE-HPLC-ESI-MSn),in order to recognize and identify the main chemical constituents in Qinghuo Zhimai tablets.Method: The possible structures of the compounds were determined according to the structure information of compounds observed from molecular ion peaks and fragment ions in HPLC-ESI-MSn negative ion mode and by comparing with literature data or control samples.Result: Through the comparative analysis on Qinghuo Zhimai Tablets and components of its formula,39 chemical constituents were identified,including 7 caffeoylquinic acids,7 iridoids,6 diterpenoid lactones,5 homoisoflavonoids,13 steroidal saponins and 1 flavone glycoside.Conclusion: This study provides a simple and rapid method for identifying chemical components in Qinghuo Zhimai tablets.

关键词(KeyWords): 高效液相色谱-电喷雾串联质谱;固相萃取;清火栀麦片;化学成分鉴定
HPLC-ESI-MSn;SPE;Qinghuo Zhimai tablets;identification of chemical constituents

Abstract:

Keywords:

基金项目(Foundation): 中医药分析技术创新研究平台建设项目;; 北京市中药基础与新药研究重点实验室项目

作者(Author): 张加余;乔延江;高晓燕;张倩;马群;卢建秋;
ZHANG Jia-yu1,2,QIAO Yan-jiang1*,GAO Xiao-yan2,ZHANG Qian1,MA Qun1,LU Jian-qiu2*(1.School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 100102,China; 2.Center of Scientific Experiment,Beijing University of Chinese Medicine,Beijing 100029,China)

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享