中国中药杂志

2013, v.38(14) 2318-2320

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UPLC-Q-TOF/MS分析橘核盐制前后成分差异
Analysis on component difference in Citrus reticulata before and after being processed with salt by UPLC-Q-TOF/MS

曾锐;付娟;武拉斌;黄林芳;
ZENG Rui1,FU Juan2,3,WU La-bin2,HUANG Lin-fang2(1.National Medical Academy,Southwest Univerisity of Nationalities,Chengdu 610041,China; 2.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China; 3.College of Chinese Medicinal Material,Jilin Agricultural University,Changchun 130118,China)

摘要(Abstract):

采用超高效液相色谱串联四级杆飞行时间质谱(UPLC-Q-TOF/MS)技术对橘核及其盐制品的成分进行分析,并比较盐制前后成分变化。通过主成分分析法和正交偏最小二乘判别法分析橘核盐制前后指纹图谱的差异,盐制品中圣草枸橼苷、柠檬苦素、诺米林、黄柏酮含量升高,发现潜在的化学标记物,鉴定为柠檬苦素、黄柏酮、诺米林,可作为区分生品与炮制品的指标成分。
To analyze components of Citrus reticulata and salt-processed C.reticulata by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF/MS),and compared the changes in components before and after being processed with salt.Principal component analysis(PCA) and partial least squares discriminant analysis(OPLS-DA) were adopted to analyze the difference in fingerprint between crude and processed C.reticulata,showing increased content of eriocitrin,limonin,nomilin and obacunone increase in salt-processed C.reticulata.Potential chemical markers were identified as limonin,obacunone and nomilin,which could be used for distinguishing index components of crude and processed C.reticulata.

关键词(KeyWords): 橘核;盐制橘核;超高效液相色谱串联四级杆飞行时间质谱
Citrus reticulata;salt-processed Citrus reticulata;UPLC-Q-TOF/MS

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(30801519,81274013);; 国家“重大新药创制”科技重大专项(2011ZX09307-002-01);; 人事部留学人员择优项目(2009-2011);; 教育部长江学者和创新团队发展计划项目(IRT1150)

作者(Author): 曾锐;付娟;武拉斌;黄林芳;
ZENG Rui1,FU Juan2,3,WU La-bin2,HUANG Lin-fang2(1.National Medical Academy,Southwest Univerisity of Nationalities,Chengdu 610041,China; 2.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China; 3.College of Chinese Medicinal Material,Jilin Agricultural University,Changchun 130118,China)

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