中国中药杂志

2005, (21)

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呼畅清肺浓缩丸对肺炎支原体感染鼠肺组织钙黏附分子表达的研究
Effect of Huchang Qingfei concentrated pellets on E-cadherin expression in lung tissue of mice infected with Mycoplasma pneumoniae

王伟明,李继昌,张洪娟,杨阳,杨桂英
WANG Wei-ming~1,LI Ji-chang~(1,2),ZHANG Hong-juan~1,YANG yang~1,YANG Gui-ying~1(1.Hei Longjiang Academy of Traditional Chinese Medicine Research Institute,Harbin 150036,China;2.Animal Medicine College,Northeast Agricultural University,Harbin 150030,China)

摘要(Abstract):

目的:探讨呼畅清肺浓缩丸对肺炎支原体感染鼠肺组织钙黏附分子表达的影响。方法:采用滴鼻吸入法复制小鼠肺炎支原体感染模型,应用透射电镜法和免疫组织化学法观察各试验组肺组织变化及钙黏附分子的表达情况。结果:电镜观察模型组肺脏细胞膜破裂,线粒体变性,嵴断裂;呼畅给药组明显改善,基本恢复正常。免疫组织化学结果,模型组肺上皮细胞膜和细支气管周围有棕黄色均匀、连续线型分布的上皮钙黏附素(E-cd)表达产物;呼畅给药组肺泡上皮细胞中E-cd的阳性细胞明显减少,呈不连续分布,表达强度显著减弱,细胞界限较清楚。与模型组比较,其表达率显著下降。结论:呼畅清肺浓缩丸能抑制肺炎支原体感染鼠肺组织中钙黏附分子的表达,对防止肺脏损害具有一定的作用。
Objective: To investigate the effects of Huchang Qingfei concentrated pellets on the expression of E-cadherin(E-cd) in the lung tissue from mice infected with Mycoplasma pneumoniae(MP).Method: A mice model of Mycoplasmal pneumonia(MPP) was developed by repeatedly intranasal infectious route.Transmission electronic microscope(TEM) and immunohistochemistry stain were performed to observe the pathological changes and expression of E-cd in lung tissues.Result: Under TEM it was found that the cellular membrane was ruptured,mitochondria was denatured,crista was broken in the pulmonary cells of the model group;the all above parameters in Huchang medicated group were improved obviously.The immunohistochemistry test showed that strong positive brown stain of E-cd expression was found in the pulmonary epithelial cell membrane and bronchial periphery in the model group,however,in the medicated group,the E-cd expression level in the cellular membrane was decreased and the expression ratio was dropped significantly as compared with the model controls.Conclusion: Huchang Qingfei concentrated pellets can inhibit the overexpression of E-cd in the lung tissue of mice with MP-infection,which may be helpful for prevention and treatment of pulmonary injury caused by MPP.

关键词(KeyWords): 呼畅清肺浓缩丸;肺炎支原体;电镜;免疫组化;黏附分子;小鼠
Huchang Qingfei concentrated pellets;Mycoplasma pneumoniae;transmission election microscope;immunohistochemistry stain;adhesion molecules;mouse

Abstract:

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基金项目(Foundation): 国家中医药管理局新药开发专项基金(DIX055B)

作者(Author): 王伟明,李继昌,张洪娟,杨阳,杨桂英
WANG Wei-ming~1,LI Ji-chang~(1,2),ZHANG Hong-juan~1,YANG yang~1,YANG Gui-ying~1(1.Hei Longjiang Academy of Traditional Chinese Medicine Research Institute,Harbin 150036,China;2.Animal Medicine College,Northeast Agricultural University,Harbin 150030,China)

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