中国中药杂志

2017, v.42(24) 4727-4732

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银杏二萜内酯K抗血小板聚集及神经保护作用研究
Effects of ginkgolide K on platelet aggregation activity and neuroprotection

陶晓倩;曹泽彧;曹亮;萧伟;
TAO Xiao-qian;CAO Ze-yu;CAO Liang;XIAO Wei;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process,Jiangsu Kanion Parmaceutical Co.,Ltd.;

摘要(Abstract):

观察不同浓度的银杏二萜内酯K(GK)对血小板活化因子(PAF)诱导血小板聚集的拮抗作用及对缺血再灌注损伤细胞和动物模型的神经保护作用。制备GK家兔含药血清,用血小板聚集功能测定法观察GK含药血清对PAF诱导的家兔血小板聚集的影响。建立缺糖缺氧复氧(OGD/R)细胞模型,采用Hoechst/PI双染考察不同浓度的GK对OGD/R损伤的SHSY5 Y细胞凋亡的影响;建立大鼠局灶性脑缺血再灌注模型(I/R),检测GK对神经行为评分及脑梗死体积的影响。GK能剂量依赖性的抑制PAF诱导的血小板聚集,逆转OGD/R损伤造成的细胞凋亡并改善大鼠脑缺血再灌注损伤后的神经行为评分及脑组织梗死体积。GK能抑制PAF诱导的血小板聚集、改善脑缺血再灌注神经损伤。
To investigate the antagonism effects of different concentrations of ginkgolide K( GK) on platelet activating factor(PAF)-induced platelet aggregation and neuroprotective effect on cells and animal models of ischemia-reperfusion injury. GK-containing serum in rabbit was prepared,and the effects of GK-containing serum on PAF-induced platelet aggregation was observed by platelet aggregation assay. The effect of different concentrations of GK on apoptosis of SH-SY5 Y cells injured by oxygen-glucose deprivation/reoxygenation( OGD/R) was investigated by Hoechst 33342/PI double staining in OGD/R cell model. The focal cerebral ischemiareperfusion model( I/R) was established in rats to detect the effects of GK on neurobehavioral scores and cerebral infarction volume.GK could inhibit PAF-induced platelet aggregation,reverse the apoptosis induced by OGD/R injury and improve the neurobehavioral score and cerebral infarction volume after cerebral ischemia-reperfusion injury in rats in a dose-dependent manner. GK can inhibit PAFinduced platelet aggregation and improve nerve injury after cerebral ischemia-reperfusion.

关键词(KeyWords): 银杏二萜内酯K;血小板活化因子;细胞凋亡;缺血再灌注
ginkgolide K;platelet activating factor;apoptosis;ischemia reperfusion

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2013ZX09402203);; 江苏省科技成果转化项目(BA2010109)

作者(Author): 陶晓倩;曹泽彧;曹亮;萧伟;
TAO Xiao-qian;CAO Ze-yu;CAO Liang;XIAO Wei;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process,Jiangsu Kanion Parmaceutical Co.,Ltd.;

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