中国中药杂志

2014, v.39(24) 4822-4828

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不同品种不同生长期桑叶中生物碱类与黄酮类化学成分的积累动态分析评价
Dynamic analysis of alkaloids and flavonoids in Genus Morus L.in China during different harvesting time

张丽丽;白永亮;宿树兰;欧阳臻;刘利;郭盛;钱大玮;段金廒;
ZHANG Li-li;BAI Yong-liang;SU Shu-lan;OU-YANG Zhen;LIU Li;GUO Sheng;QIAN Da-wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine Jiansu Key Laboratory for High Technology Research of TCM Formalae;College of Pharmacy,Jiangsu University;Sericultural Institute,Chinese Academy of Agricultural Sciences;

摘要(Abstract):

对不同品种、不同生长期的11个品种88个桑叶样品中生物碱类、黄酮类资源性化学成分的积累动态进行分析评价,以期探讨桑叶的适宜采收期及桑叶的资源化利用途径。采用UPLC-TQ/MS联用技术对桑叶中的生物碱类、黄酮类成分进行分析评价。结果表明,不同品种、不同生长期桑叶中的生物碱类、黄酮类成分含量差异较大,随着物候期的顺延,生物碱类成分的含量呈总体下降趋势,10月份后大幅下降;黄酮类成分的含量变化较小,10月份之后亦呈下降趋势。广东桑中黄酮类成分远高于生物碱类成分,而鸡桑则相反;华桑和蒙桑中生物碱类成分在7-8月间迅速下降;白桑中的生物碱类、黄酮类成分含量均处于中等水平。综合分析,药用桑叶的适宜采收期在9月底10月初。研究结果为桑叶的合理采收及资源化利用提供了科学依据。
To investigate the optimum harvesting time and utilization of mulberry leaves during different growth periods based on the content of alkaloids and flavonoids,88 samples of 11 species of mulberry leaves were collected and analyzed.UPLC-TQ / MS method was applied and the results showed that the ingredients of alkaloids and flavonoids in mulberry leaves are quite different in different growth periods and different species.There was a sharp decline of the average content of alkaloids in all samples from October,while the content of flavonoids dropped either from October but with less volatile.The content of flavonoids in M.atropurpurea was much higher than alkaloids,while M.australis was opposite completely.There was a sharp decline of alkaloids in M.cathayana and M.mongolica from July to August,however,the content of alkaloids and flavonoids in M.alba is neither too high nor too low.In summary,it is more suitable to harvest tender mulberry leaves harvested from the end of September to beginning of October that provide a scientific evidence for rational harvest and comprehensive utilization of mulberry leaves.

关键词(KeyWords): 桑叶;生物碱类;黄酮类;积累动态
Mulberry leaf;alkaloids;flavonoids;dynamic analysis

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基金项目(Foundation): 国家自然科学基金项目(81373889)

作者(Author): 张丽丽;白永亮;宿树兰;欧阳臻;刘利;郭盛;钱大玮;段金廒;
ZHANG Li-li;BAI Yong-liang;SU Shu-lan;OU-YANG Zhen;LIU Li;GUO Sheng;QIAN Da-wei;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine Jiansu Key Laboratory for High Technology Research of TCM Formalae;College of Pharmacy,Jiangsu University;Sericultural Institute,Chinese Academy of Agricultural Sciences;

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