钟应淮;奉建芳;夏明艳;魏鑫华;吴秋焱;李东勲;刘雪梅;张国松;
ZHONG Ying-huai;FENG Jian-fang;XIA Ming-yan;WEI Xin-hua;WU Qiu-yan;LI Dong-xun;LIU Xue-mei;ZHANG Guo-song;Jiangxi University of Traditional Chinese Medicine;College of Pharmacy,Guangxi University of Traditional Chinese Medicine;National Pharmaceutical Engineering Center For Solid Preparation in Chinese Herbal Medicine;

• 1:江西中医药大学
• 2:广西中医药大学药学院
• 3:中药固体制剂制造技术国家工程研究中心

摘要(Abstract)：

建立一种茶芎苯酞类有效部位(phthalide target areas of Chaxiong,CPTA)及其β-CD包合物中5种成分在大鼠血浆中药物浓度的测定方法,明确该5种成分在生物体内的药动学参数、绝对生物利用度及CPTA/β-CD包合物的相对生物利用度。采用UPLC-MS/MS测定洋川芎内酯A、正丁基苯酞、新蛇床内酯、Z-藁本内酯和丁烯基苯酞5种成分的大鼠血药浓度。色谱条件为Shim-pack GIST C_(18)-AQ HP柱(2.1 mm×100 mm,3μm),流动相0.1%甲酸水(A)-乙腈(B),梯度洗脱,流速0.3 mL·min~(-1),柱温35℃,进样量2μL;质谱条件为电喷雾离子源(ESI),正离子模式,多反应监测;采用研磨法制备CPTA/β-CD包合物,DAS 2.0软件对数据进行模型拟合,并计算CPTA的绝对生物利用度及包合物的相对生物利用度。最终成功建立了CPTA中洋川芎内酯A、正丁基苯酞、新蛇床内酯、Z-藁本内酯和丁烯基苯酞5种成分在大鼠血浆中的含量测定方法,各成分在各自范围内线性关系良好,r>0.99;这5种成分在大鼠体内的绝对生物利用度分别为22.30%,16.32%,21.90%,10.16%,12.43%,制备成CPTA/β-CD包合物后,5种成分的相对生物利用度分别为138.69%,198.39%,218.01%,224.54%,363.55%,生物利用度显著提高。该方法快速、准确,灵敏度较高,适合于中药提取物及其制剂的药动学研究。
This study aims to establish a method for the determination of the concentration of five main components of phthalide target areas of Chaxiong(CPTA) and its inclusion of β-CD in the plasma of rats, and determine the pharmacokinetic parameters, absolute bioavailability and relative bioavailability of CPTA/β-CD inclusion compound in vivo. The plasma concentrations of senkyunolide A, N-butylphthalide, new osthol lactone, Z-ligustilide and butenyl phthalide were determined with UPLC-MS/MS. The content determination was conducted at the chromatographic conditions as follows: Shim-pack GIST C_(18)-AQ HP column(2.1 mm×100 mm, 3 μm), mobile phase of 0.1% formic acid solution(A)-acetonitrile(B), gradient elution, flow rate of 0.3 mL·min~(-1), column temperature of 35 ℃ and injection volume of 2 μL. The mass spectra were obtained with electrospray ion source(ESI), positive ion mode and multi reaction monitoring. CPTA/β-CD inclusion compound was prepared by grinding method, DAS 2.0 software was used to model the data, and the absolute bioavailability of CPTA and relative bioavailability of inclusion compound were calculated. Finally, the methods for the determination of five components of senkyunolide A, N-butylphthalide, new osthol lactone, Z-ligustilide and butenyl phthalide in CPTA, were successfully established. The linear relationship among the five components was good within their respective ranges, r>0.99. The absolute bioavailability of the five components in rats was 22.30%, 16.32%, 21.90%, 10.16% and 12.43%, respectively. After CPTA/β-CD inclusion was prepared, the relative bioavailability of the five components was 138.69%, 198.39%, 218.01%, 224.54% and 363.55%, respectively, significantly improved. This method is rapid, accurate and sensitive, so it is suitable for the pharmacokinetic study of extracts in traditional Chinese medicine and their preparations.

关键词(KeyWords)： 茶芎;有效部位;UPLC-MS/MS;药代动力学;洋川芎内酯A;新蛇床内酯;Z-藁本内酯
Lgusticum sinense Oliv.cv.Chaxiong;target area;UPLC-MS/MS;pharmacokinetic;senkyunolide A;new osthol lactone;Z-ligustilide

Abstract:

Keywords:

基金项目(Foundation): 江西省重点研发计划项目(20171ACH80003);; 江西省主要学科学术和技术带头人项目(20182BCB22023);; 国家自然科学基金项目(81660667);; 江西省中药学一流学科专项科研基金项目(JXSYLXK-ZHYAO051)

作者(Author): 钟应淮;奉建芳;夏明艳;魏鑫华;吴秋焱;李东勲;刘雪梅;张国松;
ZHONG Ying-huai;FENG Jian-fang;XIA Ming-yan;WEI Xin-hua;WU Qiu-yan;LI Dong-xun;LIU Xue-mei;ZHANG Guo-song;Jiangxi University of Traditional Chinese Medicine;College of Pharmacy,Guangxi University of Traditional Chinese Medicine;National Pharmaceutical Engineering Center For Solid Preparation in Chinese Herbal Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20201012.201

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