中国中药杂志

2020, v.45(01) 133-141

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一测多评法同步测定何首乌中4种蒽醌类成分的含量
Simultaneous determination of four anthraquinones in Polygonum multiflorum by QAMS

侯文文;石燕红;宋嬿;张玉莲;瞿光航;王峥涛;杨莉;
HOU Wen-wen;SHI Yan-hong;SONG Yan;ZHANG Yu-lian;QU Guang-hang;WANG Zheng-tao;YANG Li;Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine;the MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine;Institute of Traditional Chinese Medicine International Standardization, Shanghai University of Traditional Chinese Medicine;SPH Hua Yu Chinese Herb

摘要(Abstract):

采用"一测多评"(QAMS)法,以大黄素为内参物,计算相对校正因子,建立何首乌药材中大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚-8-O-β-D-葡萄糖苷、大黄素甲醚和大黄素4种蒽醌类成分的"一测多评"质量控制方法,实现何首乌药材中游离蒽醌和结合蒽醌同步检测与定量分析。同时,采用QAMS和外标法(ESM)对30批来源不同产地的何首乌药材和12批何首乌饮片中上述4种蒽醌类成分进行含量测定,2种方法所得结果进行统计分析,结果表明2种方法之间无显著性差异。此外,比对《中国药典》中何首乌药材含量测定中以总蒽醌与游离蒽醌差值方法计算测定结合蒽醌含量的结果,QAMS法直接测定结合蒽醌含量结果更接近于外标法测定值结果。因此,该研究以大黄素为内参物建立的4种蒽醌类成分同时测定的一测多评法,方法准确度高,简便可行,可实现同时测定何首乌中主要游离蒽醌和结合蒽醌含量,有效控制何首乌药材的内在质量,对于优化提升药典中何首乌结合蒽醌含量的测定方法,具有良好的可行性。
A simple, specific and selective quantitative analysis of multi-components by single marker(QAMS) method for simultaneous determination of anthraquinones and anthraquinone glycosides in Polygonum multiflorum was developed. Four main anthraquinones and its glycosides, emodin, emodin-8-O-β-D-glucoside, physcion and physcion-8-O-β-D-glucoside were selected as analytes to evaluate the quality of P. multiflorum. Emodin was used as the internal standard, and the relative correction factors(RCFs) between emodin and the other three anthraquinones were calculated. Comparison of the contents of the four components in 30 batches of P. multiflorum from different regions and 12 batches decoction pieces from different manufacturers by QAMS and external standard method(ESM) showed that there was no significant difference between QAMS and ESM for quantification of the four main components by using relative error results, and the QAMS method was accurate and reliable, and had a good repeatability. In addition, compared with the results calculated by the difference method between total anthraquinone and free anthraquinone in the content determination of P. multiflorum in Chinese Pharmacopoeia, the results of direct determination combined anthraquinone by QAMS were very close to that by measured the external standard method. Therefore, simultaneous quantification of four main anthraquinones by using QAMS is suitable to evaluate the quality of P. multiflorum. Then the optimized assay method of the combined anthraquinone contents showed simple and feasible, which could be replaced and improved the quantification method of the combined anthraquinone in the current Chinese Pharmacopeia.

关键词(KeyWords): 何首乌;一测多评法;蒽醌类成分;结合蒽醌;相对校正因子
Polygonum multiflorum;QAMS;anthraquinones;anthraquinone glycosides;relative correction factors

Abstract:

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基金项目(Foundation): 国家中药标准化项目(ZYBZH-Y-SH-38)

作者(Author): 侯文文;石燕红;宋嬿;张玉莲;瞿光航;王峥涛;杨莉;
HOU Wen-wen;SHI Yan-hong;SONG Yan;ZHANG Yu-lian;QU Guang-hang;WANG Zheng-tao;YANG Li;Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine;the MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine;Institute of Traditional Chinese Medicine International Standardization, Shanghai University of Traditional Chinese Medicine;SPH Hua Yu Chinese Herb

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