中国中药杂志

2017, v.42(23) 4649-4655

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

广藿香对湿阻中焦证大鼠胃肠功能的影响
Effects of Pogostemon cablin on gastrointestinal function of rats with syndrome of damp retention in middle-jiao

徐雯;王楠;丁浩然;徐晶晶;瞿轶慧;蒲丹;修彦凤;
XU Wen;WANG Nan;DING Hao-ran;XU Jing-jing;QU Yi-hui;PU Dan;XIU Yan-feng;School of Pharmacy,Shanghai University of Traditional Chinese Medicine;

摘要(Abstract):

为了探讨广藿香对于湿阻中焦证大鼠胃肠功能的影响及其作用机制。通过对湿阻中焦证大鼠的胃内残留率、小肠推进率、胃液量、胃液酸度和胃蛋白酶的测定以及对胃肠组织形态的观察,评价广藿香对胃肠功能的影响;采用ELISA法检测湿阻中焦证大鼠血清中胃肠激素MTL,GAS,VIP及血清细胞因子TNF-α,IL~(-1)0的浓度,实时荧光定量PCR技术检测大鼠胃和结肠组织中水通道蛋白AQP3,AQP4,AQP8的表达,探究广藿香对于胃肠功能产生影响的作用机制。结果表明,中、高剂量广藿香(3.24,6.48 g·kg~(-1))可以明显降低大鼠的胃内残留率,促进胃排空,增加小肠推进率(P<0.05),加快胃肠推进运动,增加胃液的分泌和酸度,提高胃蛋白酶的活性,改善胃肠道损伤;不同剂量的广藿香可以升高血清中MTL和GAS的浓度,降低VIP,TNF-α,IL~(-1)0的浓度,下调胃和结肠AQP3的表达,上调结肠AQP4和AQP8的表达,中、高剂量的广藿香调节效果较好。所以广藿香可能通过调节胃肠激素、炎症细胞因子和水通道蛋白的水平达到保证胃肠功能正常的目的,该研究结果为阐明采用芳香化湿药治疗湿困中焦、运化失职适应证的作用机制奠定了基础。
To investigate the effects of Pogostemon cablin(patchouli) on gastrointestinal function of rats with the syndrome of damp retention in middle-jiao,and explore its therapeutic mechanism. In this study,gastrointestinal function of rats with the syndrome of damp retention in middle-jiao was evaluated by multiple assays including gastric remnant rate,small intestine propelling rate,gastric juice quantity,pepsin activity and gastrointestinal tissue morphology. ELISA was used to detect gastrointestinal hormones including MTL,GAS,VIP and cytokines including TNF-α and interleukin 10 in rat serum. Real-time fluorescent quantitative PCR technique was used to detect relative mRNA expression of AQP3,AQP4 and AQP8 in gastric and colonic tissues to explore the mechanism of P. cablin in treatment of gastrointestinal functions.The results showed that middle and high dose of P. cablin(3. 24,6. 48 g·kg~(-1)) could obviously decrease the gastric remnant rate,promote gastric emptying,increase the small intestine propelling rate(P < 0. 05),speed up the propulsive movement of gastrointestinal tract,increase the secretion and acidity of gastric juice,increase the activity of pepsin,and improve the injury of gastrointestinal tissue. All the doses of P. cablin could increase the concentration of MTL and GAS in serum,reduce the concentration of VIP,TNF-α and IL~(-1)0 in serum,decrease the mRNA expression of AQP3 in gastric and colonic tissues,and increase the expression levels of AQP4 and AQP8 in colonic tissues. The regulatory effects were better in middle and high dose groups. In conclusion,regulation of the levels of gastrointestinal hormones,inflammatory cytokines and aquaporins may be the paths for P. cablin to maintain normal gastrointestinal function of rats with the syndrome of damp retention in middle-jiao. The results of the study laid a foundation for clarifying the treatment mechanism of aromatic damp-resolving drugs for indications including damp retention in middle-jiao and transformation failure of spleen.

关键词(KeyWords): 广藿香;湿阻中焦证;胃肠功能;作用机制
Pogostemon cablin;syndrome of damp retention in middle-jiao;gastrointestinal function;action mechanism

Abstract:

Keywords:

基金项目(Foundation): 国家中医药行业科研专项(201507002);; 上海市大学生创新计划活动项目(2017SHUTCM014)

作者(Author): 徐雯;王楠;丁浩然;徐晶晶;瞿轶慧;蒲丹;修彦凤;
XU Wen;WANG Nan;DING Hao-ran;XU Jing-jing;QU Yi-hui;PU Dan;XIU Yan-feng;School of Pharmacy,Shanghai University of Traditional Chinese Medicine;

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享