中国中药杂志

2016, v.41(01) 56-59

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不同发芽阶段对黄芩种苗生物量及活性成分含量变化的研究
Influence of different germination stage on plant biomass and active compounds of Scutellaria baicalensis germchit

刘伟;杨帆;吕海花;周洁;王晓;耿岩玲;
LIU Wei;YANG Fan;LV Hai-hua;ZHOU Jie;WANG Xiao;GENG Yan-ling;Key Laboratory of Traditional Chinese Medicine Quality Control Techlogy,Shandong Analysis and Test Center;

摘要(Abstract):

该文主要研究从萌发到移栽5个不同发芽阶段对黄芩种苗生物量及活性成分含量的影响。采集不同发芽阶段的黄芩种苗,测定其根长、芽长、根径及根重,利用HPLC测定根中的黄芩苷、野黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的含量。结果表明,随着黄芩种苗的萌发,黄芩的各个生物学指标均升高,但根部折干率从32.90%下降至27.96%。野黄芩苷和黄芩素的含量均呈现先增加后减少的趋势,在芽/根值为0.35时达到最大,质量分数分别为3.22,3.89 mg·g~(-1),黄芩苷含量在芽/根值为0.23时最高,质量分数为107.39 mg·g~(-1),汉黄芩苷在芽/根值为0.06时含量最高,质量分数为16.11 mg·g~(-1),汉黄芩素则呈现逐渐增加的趋势,在芽/根值为0.50时达到最大值,质量分数为0.88 mg·g~(-1)。当芽/根值为0.35时,黄芩种苗中的野黄芩苷、黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的含量达到或接近发芽阶段的最大值,可用芽/根值作为黄芩种苗活性成分的判定指标。
This paper is aimed to study plant biomass and active compounds of Scutellaria baicalensis germchit in different five stages( from germination to transplant). The length of shoot and root,the diameter and the weight of root were determined. HPLC method was used to determine the content of active compounds( baicalin,scutellarin,wogonoside,baicalein,wogonin). According to the results,various biological indicators increased with the germination of seedling. However,the drying rate of the root declined to 27. 96%from 32. 90%. The contents of scutellarin and baicalein increased firstly,and then decreased. The maximums of them were 3. 22,3. 89mg·g~(-1) while the data of shoot / root was 0. 35. The maximums of the contents of baicalin and wogonoside were 107. 39,16. 11 mg·g~(-1)while the data of shoot / root was 0. 23 and 0. 06. The contents of wogonin gradually increased to the maximum of 0. 88 mg·g~(-1)while the data of shoot / root was 0. 50. In conclusion,the contents of baicalin,scutellarin,wogonoside,baicalein and wogonin reached or approached the maximum at germination stage while the data of shoot / root was 0. 35. The rate of shoot and root can be used as a judging index of active compounds for S. baicalensis germchit.

关键词(KeyWords): 黄芩;发芽阶段;生物量;活性成分含量
Scutellaria baicalensis;germination stage;biomass;the content of active compounds

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作者(Author): 刘伟;杨帆;吕海花;周洁;王晓;耿岩玲;
LIU Wei;YANG Fan;LV Hai-hua;ZHOU Jie;WANG Xiao;GENG Yan-ling;Key Laboratory of Traditional Chinese Medicine Quality Control Techlogy,Shandong Analysis and Test Center;

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