中国中药杂志

2021, v.46(02) 306-311

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以线粒体为靶点筛选中药抗肝毒性的活性成分研究思路与方法
Research ideas and method on screening active components of traditional Chinese medicine against hepatotoxicity with mitochondria as target

王玉琳;柳雨亭;陶艳艳;刘成海;瞿惠燕;周华;杨涛;
WANG Yu-lin;LIU Yu-ting;TAO Yan-yan;LIU Cheng-hai;QU Hui-yan;ZHOU Hua;YANG Tao;Institute of Cardiovascular Disease, Department of Cardiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;Shanghai Key Laboratory of Traditional Chinese Clinical Medicine;Institute of Liver Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine;

摘要(Abstract):

肝脏是药物代谢的主要场所,肝细胞线粒体是药物肝损伤的重要靶标,线粒体自噬可以维持细胞内线粒体的健康运转以及细胞的稳定增殖,因此利用线粒体自噬来清除受损线粒体是抗药物性肝损伤的重要策略。多种中药中含有可以增强线粒体自噬的活性成分,可以调节线粒体自噬从而缓解相关疾病。但如何准确高效地从中药中发现并评价此类对线粒体有靶向活性成分的研究方法鲜有报道。而采用液质联用技术,以体内过程结合血清药理学的方法能准确、高效地锁定作用于线粒体靶点的中药活性成分。该文综述了增加线粒体自噬解药物肝毒性的中药成分研究思路与方法,为以线粒体为靶点的中药活性成分研究提供新思路与方法。
Liver is the main place of drug metabolism. Mitochondria of hepatocytes are important targets of drug-induced liver injury. Mitochondrial autophagy could maintain the healthy operation of mitochondria in cells and the stable proliferation of cells. Therefore, the use of mitochondrial autophagy to remove damaged mitochondria is an important strategy of anti-drug-induced liver injury. Active ingredients that could enhance mitochondrial autophagy are contained in many traditional Chinese medicines, which could regulate the mitochondrial autophagy to alleviate relevant diseases. However, there are only a few reports on how to accurately and efficiently identify and evaluate such components targeting mitochondria from traditional Chinese medicine. Liquid chromatography-mass spectro-metry(LC-MS) combined with serum pharmacology in vivo can be used to accurately and efficiently find active ingredients of traditional Chinese medicine acting on mitochondrial targets. This paper reviewed the research ideas and methods of traditional Chinese medicine ingredients for increasing the hepatotoxicity of mitochondrial autophagy, in order to provide new ideas and methods for the study of active ingredients of traditional Chinese medicine targeting mitochondria.

关键词(KeyWords): 线粒体;线粒体自噬;药物性肝损伤;中药;活性成分
mitochondria;mitochondrial autophagy;drug-induced liver injury;traditional Chinese medicine;active ingredients

Abstract:

Keywords:

基金项目(Foundation): 上海市医学领军人才计划项目(2015年046号);; 上海市卫计委中医药三年行动计划项目[ZY-(2018-2020)-CCCX-5001,ZY-(2018-2020)-CCCX-2003];; 上海市科委科技支撑项目(18401900700);; 上海市申康医学发展中心新兴前沿技术联合攻关项目(SHDC12018125)

作者(Author): 王玉琳;柳雨亭;陶艳艳;刘成海;瞿惠燕;周华;杨涛;
WANG Yu-lin;LIU Yu-ting;TAO Yan-yan;LIU Cheng-hai;QU Hui-yan;ZHOU Hua;YANG Tao;Institute of Cardiovascular Disease, Department of Cardiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;Shanghai Key Laboratory of Traditional Chinese Clinical Medicine;Institute of Liver Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20200904.601

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