车朋;刘久石;齐耀东;强亭燕;宋奕辰;魏雪苹;刘海涛;张本刚;
CHE Peng;LIU Jiu-shi;QI Yao-dong;QIANG Ting-yan;SONG Yi-chen;WEI Xue-ping;LIU Hai-tao;ZHANG Ben-gang;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College;

• 1:中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室

摘要(Abstract)：

建立UPLC-ELSD方法同时测定5种贝母类药材中贝母辛、西贝母碱苷、西贝母碱、贝母素乙、贝母素甲和湖贝甲素6种生物碱成分。采用Waters Acquity UPLC^(TM) CSH C_(18)柱(2.1 mm×100 mm,1.7μm);0.02%二乙胺水溶液和甲醇为流动相,梯度洗脱(0～2 min,45%A;2～5 min,45%～25%A;5～7 min,25%A;7～17 min,25%～10%A;17～20 min,10%A),流速0.3 mL·min(TM) CSH C_(18)柱(2.1 mm×100 mm,1.7μm);0.02%二乙胺水溶液和甲醇为流动相,梯度洗脱(0～2 min,45%A;2～5 min,45%～25%A;5～7 min,25%A;7～17 min,25%～10%A;17～20 min,10%A),流速0.3 mL·min^(-1),柱温35℃,样品室温度20℃,漂移管温度45℃,喷雾器参数40%,进样量为1μL,同时测定贝母类药材中6种生物碱活性成分;并采用聚类分析(HCA)和主成分分析(PCA)对不同种类贝母类药材的质量进行评价。该方法操作简单,结果准确,稳定可靠,能快速分析5种贝母类药材中6种生物碱的含量,为贝母类药材的质量控制提供了依据。
An UPLC method was established for the direct determination of six major bioactive isosteroidal alkaloids, namely peimisine, imperialine, sipeimine-3-D-glucoside, verticinone, verticine and hupehenine from the bulbus of Fritillaria(Beimu), a commonly used antitussive traditional Chinese medicinal(TCM) herb. An Acquity UPLC(-1),柱温35℃,样品室温度20℃,漂移管温度45℃,喷雾器参数40%,进样量为1μL,同时测定贝母类药材中6种生物碱活性成分;并采用聚类分析(HCA)和主成分分析(PCA)对不同种类贝母类药材的质量进行评价。该方法操作简单,结果准确,稳定可靠,能快速分析5种贝母类药材中6种生物碱的含量,为贝母类药材的质量控制提供了依据。
An UPLC method was established for the direct determination of six major bioactive isosteroidal alkaloids, namely peimisine, imperialine, sipeimine-3-D-glucoside, verticinone, verticine and hupehenine from the bulbus of Fritillaria(Beimu), a commonly used antitussive traditional Chinese medicinal(TCM) herb. An Acquity UPLC^(TM) CSH C_(18) column(2.1 mm×100 mm, 1.7 μm) was used for all analysis. The investigated six compounds were all separated with gradient mobile phase consisting of 0.02% diethylamine-water-methanol at a flow rate of 0.3 mL·min(TM) CSH C_(18) column(2.1 mm×100 mm, 1.7 μm) was used for all analysis. The investigated six compounds were all separated with gradient mobile phase consisting of 0.02% diethylamine-water-methanol at a flow rate of 0.3 mL·min^(-1). The temperature of sample manager was set at 20 ℃. Drift tube temperature was 45 ℃, and spray parameter was 40% with injection volume of 1 μL. Then, the further quality assessment of Beimu was carried out by cluster analysis(CA) and principal component analysis(PCA). The investigated all had good linearity(r≥0.998 9) over the tested ranges. The method is simple, accurate and reproducible, and can be used for determining the content of six major bioactive isosteroidal alkaloids.

关键词(KeyWords)： 贝母;UPLC-ELSD;生物碱;含量测定;统计分析
Beimu;UPLC-ELSD;alkaloids;quantitative analysis;statistical analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81573531);; 中国医学科学院与健康科技创新工程项目(2016-IZM-2-003);; 常用道地药材及其产区的特征、标准及数字化(2015FY1115000)

作者(Authors): 车朋;刘久石;齐耀东;强亭燕;宋奕辰;魏雪苹;刘海涛;张本刚;
CHE Peng;LIU Jiu-shi;QI Yao-dong;QIANG Ting-yan;SONG Yi-chen;WEI Xue-ping;LIU Hai-tao;ZHANG Ben-gang;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College;

DOI: 10.19540/j.cnki.cjcmm.20191223.201

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