向俊洁;陈娜;李化;张雪;杨滨;黄璐琦;
XIANG Jun-jie;CHEN Na;LI Hua;ZHANG Xue;YANG Bin;HUANG Lu-qi;Academician Workstation of Jiangxi University of Traditional Chinese Medicine;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;Shanghai Traditional Chinese Medicine Co.,Ltd.;China Academy of Chinese Medical Sciences;

• 1:江西中医药大学院士工作站
• 2:中国中医科学院中药研究所
• 3:上海市药材有限公司
• 4:中国中医科学院

摘要(Abstract)：

为开发利用西红花去柱头花部,采用色谱及质谱技术对其黄酮类成分进行了定性定量分析。采用UPLC-Q-TOF-MS技术对西红花去柱头花部进行了化学成分的定性分析,推测了50个黄酮醇类化合物和5个花色苷类化合物;采用UPLC技术建立了西红花去柱头花部的指纹图谱及其主要黄酮类成分山柰酚-3-O-槐糖苷、飞燕草素-3,5-二-O-葡萄糖苷的含量测定方法,并对10批不同产地样品进行了测定分析,10批样品指纹图谱相似度均在0.99以上,山柰酚-3-O-槐糖苷质量分数为44.21～58.73 mg·g~(-1),飞燕草素-3,5-二-O-葡萄糖苷质量分数为2.11～6.37 mg·g~(-1);采用电子眼技术将样品的颜色数字化,发现样品颜色与飞燕草素-3,5-二-O-葡萄糖苷的含量显著相关。研究结果显示,西红花去柱头花部富含黄酮类成分,有开发利用的潜力;不同产地样品中花色苷类成分含量的差异较大,应注意样品前处理及贮藏的方法。
In order to better utilize saffron floral bio-residues(SFB), a qualitative and quantitative analysis of flavonoids in SFB was conducted using UPLC-MS and UPLC, respectively. On the one hand, 50 flavonols and 5 anthocyanins were putatively characte-rized by using UPLC-Q-TOF-MS. On the other hand, an UPLC method was established for determining the fingerprint of SFB as well as testing the main flavonoids kaempferol-3-O-sophoroside and delphinidin-3,5-di-O-glucoside. Contents of kaempferol-3-O-sophoroside and delphinidin-3,5-di-O-glucoside of 10 batches of samples were 44.21-58.73 mg·g~(-1) and 2.11-6.37 mg·g~(-1), respectively, and the similarities of 10 batches were more than 0.99. In addition, the color of the samples was digitized by using electronic eye technology, and it was found that the color of the samples was significantly correlated with the content of delphinidin-3,5-di-O-glucoside. The richness of flavonoids in SFB indicated its potential for development and utilization, and the large variation in anthocyanin content among samples from different regions suggested that more attention should be paid to the methods of sample pretreatment and storage.

关键词(KeyWords)： 西红花去柱头花部;黄酮类成分;液质定性分析;指纹图谱;含量测定
saffron floral bio-residues;flavonoids;UPLC-Q-TOF-MS qualitative analysis;fingerprint;content determination

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划“中医药现代化研究”专项(2018YFC1706103);; 中央本级重大增减支项目(2060302)

作者(Author): 向俊洁;陈娜;李化;张雪;杨滨;黄璐琦;
XIANG Jun-jie;CHEN Na;LI Hua;ZHANG Xue;YANG Bin;HUANG Lu-qi;Academician Workstation of Jiangxi University of Traditional Chinese Medicine;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;Shanghai Traditional Chinese Medicine Co.,Ltd.;China Academy of Chinese Medical Sciences;

Email:

DOI: 10.19540/j.cnki.cjcmm.20201214.302

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