中国中药杂志

2010, v.35(06) 725-728

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茅苍术中苍术酮、茅术醇、β-桉叶醇和苍术素的同时含量测定及其聚类分析
Simultaneous determination of atractylone,hinesol,β-eudesmol,atrctylodin in Atractylodes lancea and hierarchical cluster analysis

张磊;欧阳臻;赵明;王佩香;方静;
ZHANG Lei1,OUYANG Zhen1*,ZHAO Ming1,2,WANG Peixiang1,FANG Jing1(1.School of Pharmacy,Jiangsu University,Zhenjiang 212013,China;2.Affiliated Hospital of Jiangsu University,Zhenjiang 212001,China)

摘要(Abstract):

目的:建立气相色谱法同时测定茅苍术药材中苍术酮、茅术醇、β-桉叶醇和苍术素的含量,根据含量水平对茅苍术药材进行聚类分析。方法:HP-1弹性石英毛细管色谱柱(0.25 mm×30 m,0.25μm);氢火焰离子化检测器(FID);载气氮气;起始温度145℃,保持25 min,以10℃.min-1升温至250℃,保持10 min;进样方式为分流进样,分流比为40∶1;进样量为2μL;采用SPSS 13.0统计软件进行聚类分析。结果:苍术酮、茅术醇、β-桉叶醇和苍术素进样浓度分别在0.012~2.32(r=0.999 8),0.008~1.68(r=0.999 8),0.008~1.76(r=0.999 9),0.016~3.20 g.L-1(r=0.999 7)呈良好线性关系;平均回收率(n=3)分别为98.0%~99.0%,97.7%~99.4%,98.4%~99.2%,97.8%~99.7%;所分析的茅苍术药材大致划分为两类。结论:该方法准确、简便,具有良好的重复性和稳定性,可用于茅苍术药材中苍术酮、茅术醇、β-桉叶醇和苍术素含量的同时测定;道地产区茅苍术与其它非道地产区茅苍术4种活性成分含量的差异较大。
Objective: To develop a GC method for simultaneous determination of 4 compounds(atractylone,hinesol,β-eudesmol and atrctylodin) in Atractylodes lancea.Method: A HP-1 capillary column(0.25 mm×30 m,0.25 μm)was used;The detector was FID:Inlet temperature was 250 ℃;The detector temperature was 250 ℃;The column temperature was set at 145 ℃ and held for 25 min after injection,then programmed at 10 ℃·min-1 to 250 ℃ and held for 10 min at the temperature.The carrying gas was nitrogen,split ratio was 40∶1,Injection volume was 2 μL,Cluster analysis was performed by SPSS13.0 software.Result: The linear ranges for atractylone,hinesol,β-eudesmol and atrctylodin were 0.012-2.32(r=0.999 8),0.008-1.68(r=0.999 8),0.009-1.76(r=0.999 9),0.016~3.20 g·L-1(r=0.999 7),respectively.The average recoveries(n=3) of atractylone,hinesol,β-eudesmol and atrctylodin were 98.0%-99.0%,97.7%-99.4%,98.4%-99.2%,97.8%-99.7%,respectively.The samples analyzed were divided into two classes.Conclusion: This method is simple,specific,repeatable and stable.It can be applied for the simultaneous determination of 4 compounds(atractylone,hinesol,β-eudesmol and atrctylodin) in A.lancea,which will provide the basis for the quality control of A.lancea.The contents of 4 active compounds were significantly different between geo-authentic and non-authentic producing areas.

关键词(KeyWords): 茅苍术;苍术酮;茅术醇;β-桉叶醇;苍术素;含量测定;聚类分析
Atractylodes lancea;atractylone;hinesol;β-eudesmol;atrctylodin;determination;cluster analysis

Abstract:

Keywords:

基金项目(Foundation): 江苏省中医药局中医药科技专项(HZ07005,LZ09086)

作者(Author): 张磊;欧阳臻;赵明;王佩香;方静;
ZHANG Lei1,OUYANG Zhen1*,ZHAO Ming1,2,WANG Peixiang1,FANG Jing1(1.School of Pharmacy,Jiangsu University,Zhenjiang 212013,China;2.Affiliated Hospital of Jiangsu University,Zhenjiang 212001,China)

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