中国中药杂志

2004, (04)

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

用色谱和ESI-MS方法考察四物汤提取部位的化学成分
Investigation of constituents in Siwu Tang fractions by chromatographic and ESI-MS methods

梁乾德,马百平,李卫华,张村,王红霞,张首国,魏开华,王升启
LIANG Qian de 1, MA Bai ping 1, LI Wei hua 2, ZHANG Cun 1, WANG Hong xia 2, ZHANG Shou guo 1, WEI Kai hua 2, WANG Sheng qi 1 (1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China ; 2. National Center of Biomedical Analysis, Academy of Military Medical Sciences, Beijing 100850, China )

摘要(Abstract):

目的 :为了探讨四物汤补血作用的物质基础 ,制备 3种不同极性的四物汤提取部位 (C1,C2 ,C3) ,对这3种部位的化学成分特点进行考察。方法 :综合运用高效薄层色谱 (HPTLC)、高效液相色谱 (HPLC)、电喷雾离子化质谱 (ESI MS) 3种方法对四物汤的C1,C2 ,C3部位及四味单药相应部位进行分析和比较。结果 :四物汤部位C1中含有种类丰富的成分 ,包括阿魏酸、芍药苷等 ,并且应含有藁本内酯 ,而糖类物质极少 ;C2的成分以芍药苷、单糖、二糖为主 ;C3的成分以单糖和二糖为主。结论 :综合运用色谱和直接输注ESI MS ,可以获得关于方剂提取部位组成的丰富信息。不同方法的结果可以相互比较 ,相互印证 ,使所获得的信息比单一方法更全面更确切。本研究的结果对于探讨四物汤补血物质基础具有参考意义
Objective:In order to discuss the chemical foundation of hematopoietic effect of Siwu Tang, three fractions of different polarities (C1, C2 and C3) were prepared from Siwu Tang and the characteristics of these fractions' constituents were investigated. Method:Fraction C1, C2 and C3 of Siwu Tang and corresponding fractions of Siwu Tang's four ingredient drugs were analyzed and compared, synthetically using the three methods of high performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC) and direct infusion electrospray ionization mass spectrometry (ESI MS). Result:Fraction C1 of Siwu Tang contained various types of compounds, including ferulic acid, paeoniflorin and supposedly ligustilide, etc. Saccharide content in fraction C1 was very little. The major constituents in fraction C2 of Siwu Tang were paeoniflorin, monosaccaride and disaccharide. The major constituents in fraction C3 of Siwu Tang were monosaccaride and disaccharide. Conclusion:With synthetical chromatographic and direct infusion ESI MS methods, abundant information on composition of fractions of traditional Chinese medicine formulas can be obtained. The results gained with different methods can be compared with each other and corroborate each other, so that the obtained information can be more comprehensive and more definite than that gained with single method. The results of this study are important as references for the discussion of the chemical foundation of hematopoietic effect of Siwu Tang.

关键词(KeyWords): 四物汤;成分;薄层色谱;高效液相色谱;质谱
Siwu Tang; constituent; HPTLC; HPLC; ESI-MS

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目 ( 3 0 2 71617,3 0 0 70 913 );; 军队“十五”重点项目 ( 0 1Z0 19);; 北京市“二四八”重大创新工程项目(H0 10 2 10 2 2 0 113 )

作者(Authors): 梁乾德,马百平,李卫华,张村,王红霞,张首国,魏开华,王升启
LIANG Qian de 1, MA Bai ping 1, LI Wei hua 2, ZHANG Cun 1, WANG Hong xia 2, ZHANG Shou guo 1, WEI Kai hua 2, WANG Sheng qi 1 (1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China ; 2. National Center of Biomedical Analysis, Academy of Military Medical Sciences, Beijing 100850, China )

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享