中国中药杂志

2013, v.38(19) 3287-3290

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HPLC同时测定苦碟子注射液中单咖啡酰基酒石酸、咖啡酸、绿原酸、阿魏酸、菊苣酸的含量
Simultaneous determination of five organic acids in Kudiezi injection by HPLC

刘睿;孙璐;赵雯;李遇伯;张艳军;
LIU Rui;SUN Lu;ZHAO Wen;LI Yu-bo;Zhang Yan-jun;College of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine;

摘要(Abstract):

该文目的是建立同时测定苦碟子注射液中5种有机酸类成分(单咖啡酰基酒石酸、咖啡酸、绿原酸、阿魏酸、菊苣酸)含量的HPLC分析方法。采用Diamonsil C18色谱柱(4.6 mm×200 mm,5μm),流动相乙腈-水,梯度洗脱,流速1.0 mL·min-1,柱温35℃,检测波长325 nm。单咖啡酰基酒石酸、咖啡酸、绿原酸、阿魏酸、菊苣酸在0.64~81.60,0.09~11.10,0.09~11.30,0.10~12.80,0.43~55.50 mg·L-1线性关系良好,r分别为0.999 9,0.999 8,0.999 8,0.999 9,0.999 8,平均回收率分别为101.8%,100.9%,99.24%,99.83%,101.9%,RSD不大于2.0%。该方法简单方便、重复性好、准确可靠,可为苦碟子注射液质量控制提供依据。
The aim was to develop a high performance liquid chromatography method for simultaneous determination of five organic acids in Kudiezi injection. The Diamonsil C18column( 4. 6 mm × 200 mm,5 μm) was adopted with acetonitrile and water as the mobile phase at a gradient mode program. The flow rate was 1. 0 mL·min- 1,detection wavelength was 325 nm,and column temperature was 35 ℃. The linear range of monocaffeyltartaric acid,chlorogenic acid,caffeic acid,ferulic acid,and chicoric acid were 0. 64-81. 60( r = 0. 999 9),0. 09-11. 10( r = 0. 999 8),0. 09-11. 30( r = 0. 999 8),0. 10-12. 80( r = 0. 999 9),0. 43-55. 50 mg·L- 1( r =0. 999 8),respectively. The average recoveries were 101. 8%,100. 9%,99. 24%,99. 83%,101. 9%,respectively,with RSD of less than 2. 0%. The developed HPLC method was simple,sensitive and accurate with good repeatability. This work provided helpful information for comprehensive quality control of Kudiezi injection.

关键词(KeyWords): 苦碟子注射液;有机酸类化合物;含量测定;HPLC
Kudiezi injection;organic acids;content determination;HPLC

Abstract:

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2011ZX09401-305-42);; 教育部高等学校博士学科点专项科研基金项目(20121210120001);; 天津市高等学校科技发展基金项目(20110215)

作者(Author): 刘睿;孙璐;赵雯;李遇伯;张艳军;
LIU Rui;SUN Lu;ZHAO Wen;LI Yu-bo;Zhang Yan-jun;College of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine;

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