中国中药杂志

2007, (04) 307-309

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草珊瑚总黄酮提取工艺及其含量动态变化
Study on extraction technics and variety trends of the flavonoids in Sarcandrae glabra

郁建生;郁建平;
YU Jian-sheng~(1),YU Jian-ping~(2)(1.Tongren Vocational Institute,Tongren 554300,China;2.College of Life Science,University of Guizhou,Guiyang 550025,China)

摘要(Abstract):

目的:探讨草珊瑚总黄酮的提取工艺及含量动态变化。方法:以贵州产草珊瑚为原料,采取乙醇浸提L9(34)正交试验方法优化总黄酮提取工艺,然后在该工艺条件下对自然存放时间不同、采摘季节不同、部位不同的草珊瑚原料进行总黄酮含量的动态变化考察。结果:乙醇体积分数对总黄酮提取量有显著影响,提取温度对总黄酮提取量有影响;草珊瑚自然存放时间越长,总黄酮含量越低,平均每年下降24.74%;不同季节采摘的草珊瑚其总黄酮含量以10~12月最高;经对贵州草珊瑚根、茎、叶中总黄酮的提取测定表明,草珊瑚叶中总黄酮含量最高,茎中最低。结论:以原料10倍量的60%乙醇作为溶剂、提取温度80℃、提取时间3h、提取次数3次,即可获得草珊瑚总黄酮最佳提取效果;以10~12月采摘、叶、存放时间越短,总黄酮含量越高。
Objective: To study on extraction technics and variety trends of the flavonoids in Sarcandrae glabra.Method: By using S.glabra in Guizhou as materials and the experiment of L_(9)(3~(4)) by ethanol as extracting way,The variety trends of the flavonoids from S.glabra are studied under the optimum extracting conditions during different raw stored times,different gathering season,different S.glabra position.Result: The flavonoid's contains was more low when the raw material was stored more long and descended average 24.74% annually.The flavonoid's contains had the tallest in 10-12 month by gathering indifferent season. The flavonoids contains were the tallest in the leaf of S.glabra in Guizhou than in the root and stem,and the contains were the lowest in stem.Conclusion: The optimum conditions of extracting flavonoids from S.glabra are obtained when the condition is 60% of density of ethanol at 10 times of volume,80 ℃ of extracting temperature,3 h of extracting hours,3 times of extracting number of times in 10-12 month,in the leaf,the raw material was stored more shortest,the flavonoid's contains had the tallest.

关键词(KeyWords): 草珊瑚;总黄酮;提取工艺;含量;动态变化
Sarcandrae glabra;flavonoids;extraction technics;content;variety trends

Abstract:

Keywords:

基金项目(Foundation): 贵州省省长专项资金项目[黔省专合字(2005)261号];; 贵州省“十一五”农业科技攻关项目[黔科合NY字(2006)3020]

作者(Author): 郁建生;郁建平;
YU Jian-sheng~(1),YU Jian-ping~(2)(1.Tongren Vocational Institute,Tongren 554300,China;2.College of Life Science,University of Guizhou,Guiyang 550025,China)

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