中国中药杂志

2012, v.37(02) 186-188

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土壤缺水对黄芩过氧化氢清除系统相关酶基因表达的影响
Effect of water deficit on gene expression of enzymes related with hydrogen peroxide detoxification system in Scutellaria baicalensis

伍翀;秦双双;袁媛;陈平;林淑芳;
WU Chong1,2,QIN Shuangshuang1,2,YUAN Yuan1,CHEN Ping2,LIN Shuifang2(1.Wuhan Polythechnic University,Wuhan 430023,China; 2.Institute of chinese Materia Medica,China Academy of Chinese Medicinal Sciences,Beijing 100700,China)

摘要(Abstract):

目的:分析土壤缺水对黄芩SOD,APX,DHAR,MDHAR基因表达的影响。方法:利用受控实验对黄芩进行土壤缺水胁迫;利用半定量RT-PCR对基因转录水平进行分析。结果:与对照组相比,胁迫70 d后,APX基因转录水平显著降低;胁迫30,50 d后,DHAR基因转录水平显著降低;胁迫50 d后,MDHAR1基因有显著降低。结论:在土壤缺水的条件下,维生素C(AsA)作为一种重要的抗氧化剂在过氧化氢清除系统中可能发挥主要作用,其与黄芩素积累可能处于竞争的关系。
Objective:To analysis the effects of water deficit on the transcript level of SOD,APX,DHAR and MDHAR genes in Scutellaria baicalensis.Method:Three-month-old S.baicalensis was in glasshouse under water deficit stress,and the transcript level of SOD,APX,DHAR and MDHAR genes were analysis utilized semi-quantitative RT-PCR.Result:Compared with the control group,a significant decline of the transcriptional level of APX gene was observed at 70 days after water deficit.The transcript level of DHAR gene was reduced at 30 and 50 days after water deficit.And MDHAR1 gene was significant declined at 50 days.Conclusion:AsA which is an important antioxidant plays a major role in hydrogen peroxide clear system under water deficit,and maybe have an antagonistic effect to the accumulation of baicalein.

关键词(KeyWords): 土壤水分;黄芩;抗坏血酸氧化酶;脱氢抗坏血酸还原酶
soil water;Scutellaria baicalensis;APX;DHAR

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09301-005-03);; 北京市科技新星项目(2008B82);; 中国博士后科学基金特别资助项目(201003228)

作者(Author): 伍翀;秦双双;袁媛;陈平;林淑芳;
WU Chong1,2,QIN Shuangshuang1,2,YUAN Yuan1,CHEN Ping2,LIN Shuifang2(1.Wuhan Polythechnic University,Wuhan 430023,China; 2.Institute of chinese Materia Medica,China Academy of Chinese Medicinal Sciences,Beijing 100700,China)

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