中国中药杂志

2014, v.39(10) 1822-1825

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芪苈强心胶囊中12种成分的UPLC-MS测定
Determination of twelve active compounds in Qili Qiangxin capsules by UPLC-MS

刘颖;欧阳玥;李松;刘敏彦;乔莉;赵韶华;
LIU Ying;OUYANG Yue;LI Song;LIU Min-yan;QIAO Li;ZHAO Shao-hua;Heilongjiang University of Traditional Chinese Medicine;Yiling Medical Research Institute of Hebei Province,the State Administration of Traditional Chinese Medicine Key Laboratory(Heart and Cerebral Vesselscollateraldisease);Department of Collateral Theory,Combination of Traditional Chinese Medicinewith Western Medicine Academy of Hebei,Key Laboratory of Network Disease of Hebei Province;

摘要(Abstract):

该文建立了芪苈强心胶囊中12种有效成分的超高效液相色谱结合质谱(UPLC-MS)的定量方法。采用Phenomenex UPLC Kinetex C18色谱柱(2.1 mm×100 mm,2.6μm),以乙腈-0.1%甲酸溶液为流动相梯度洗脱,流速0.4 mL·min-1,柱温40℃,进样量5μL,质谱在负离子模式下采用TOF-MS及TOF-MS/MS方法检测。结果表明,12种有效成分毛蕊异黄酮-7-O-葡萄糖苷、异槲皮苷、柚皮芸香苷、橙皮苷、人参皂苷Re、人参皂苷Rg1、杠柳毒苷、人参皂苷Rf、人参皂苷Rb1、黄芪甲苷、人参皂苷Rd、杠柳苷H1在检测的范围内线性良好,R2均大于0.999 0,方法回收率为98.0%~102%,RSD均小于3.9%。该方法简便快速、准确度高,可用于芪苈强心胶囊的质量控制。
In order to establishan UPLC-MS method for determination of twelve active compoundsin Qili Qiangxin capsules including astragaloside,calycosin-7-O-glucoside,ginsenoside Rb1,ginsenoside Re,ginsenoside Rd,ginsenoside Rg1,ginsenoside Rf,periplocin,periplocoside H1,hesperidin,narirutin,isoquercitrin,the chromatographic separations were performedon a Phenomenex UPLC Kinetex C18column(2.1 mm × 100 mm,2.6 μm)with gradient elution of acetonitrile and 0.1% aqueous formic acidat a flow rate of 0.4 mL·min- 1.The temperature was set as 40 ℃ and injection volume was 5 μL.The monitoring of all analytes was achieved under the negative ionization mode with TOF-MS and TOF-MS /MS method.The twelve analytes showed good linearity( R2> 0.999 0)within the test ranges,the average recoveries were 98.0%-102%,respectively,and the RSD were less than 3.9%,respectively.The established method is simple,rapid,and sensitive,and can be used for quality control of Qili Qiangxin capsules.

关键词(KeyWords): UPLC-MS;芪苈强心胶囊;含量测定
UPLC-MS;Qili Qiangxin capsules;determination

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划(973计划)项目(2012CB518606);; 国家自然科学基金青年基金项目(81102768)

作者(Author): 刘颖;欧阳玥;李松;刘敏彦;乔莉;赵韶华;
LIU Ying;OUYANG Yue;LI Song;LIU Min-yan;QIAO Li;ZHAO Shao-hua;Heilongjiang University of Traditional Chinese Medicine;Yiling Medical Research Institute of Hebei Province,the State Administration of Traditional Chinese Medicine Key Laboratory(Heart and Cerebral Vesselscollateraldisease);Department of Collateral Theory,Combination of Traditional Chinese Medicinewith Western Medicine Academy of Hebei,Key Laboratory of Network Disease of Hebei Province;

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