中国中药杂志

2005, (06) 440-442

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注射用复方荭草(冻干粉针)指纹图谱研究
Study on RP-HPLC fingerprint of Compound Hongcao Injection

兰燕宇,刘丽娜,王永林,李勇军,黄勇,何迅,郑林,王爱民
LAN Yan-yu, LIU Li-na, WANG Yong-lin, LI Yong-jun, HUANG Yong, HE Xun, ZHENG Lin, WANG Ai-min(School of Pharmacy, Guiyang Medical Colledge, Guiyang 550004, China)

摘要(Abstract):

目的:采用RP HPLC建立注射用复方荭草(冻干粉针)的指纹图谱检测标准。方法:用Inersil ODS3色谱柱,乙腈0.1%磷酸溶液梯度洗脱;流速1.0mL·min1;检测波长300nm。结果:在相同色谱条件下获得药材、中间体和制剂的色谱图各色谱峰分离较好,制剂与药材、中间体的指纹图谱有良好的相关性,达到指纹图谱的技术要求。结论:指纹图谱17个共有峰的相对保留时间及峰面积比值,可作为注射用复方荭草(冻干粉针)质量控制的重要依据之一。
Objective: To establish the fingerprint detecting standard of Compound Hongcao Injection by reversed phase high performance liquid chromatography. Method: The chromatographic conditions were as follows: an Inersil-ODS-3 column was used; the mobile phases (acetontrile and 0.1% phosphoric acid) with gradient elution; the flow rate 1.0 mL·mim~(-1) and the UV absorbance detection at 300nm. Result: Under the same selected chromatographic conditions, the HPLC fingerprints of raw drugs and their intermediate, finished products were obtained with good separation and correlation according to the technical requirements of fingerprint on Injection of Chinese traditional medicine. Conclusion: 17 common peaks as well as their retention times and peak area ratios on HPLC fingerprint can be used as the important parameters of the quality control for Compound Hongcao Injection.

关键词(KeyWords): 注射用复方荭草(冻干粉针);荭草;高效液相色谱;指纹图谱
Compound Hongcao Injection; Herba Polygoni Orientalis; HPLC; fingerprint

Abstract:

Keywords:

基金项目(Foundation): 贵州省中药现代化科技产业研究开发专项基金项目(黔科合中药专字[2004]03号)

作者(Author): 兰燕宇,刘丽娜,王永林,李勇军,黄勇,何迅,郑林,王爱民
LAN Yan-yu, LIU Li-na, WANG Yong-lin, LI Yong-jun, HUANG Yong, HE Xun, ZHENG Lin, WANG Ai-min(School of Pharmacy, Guiyang Medical Colledge, Guiyang 550004, China)

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