江美芳;王勤辉;庞欢欢;田悦;王晓烨;孙璐;高伟;黄建梅;
JIANG Mei-fang;WANG Qin-hui;PANG Huan-huan;TIAN Yue;WANG Xiao-ye;SUN Lu;GAO Wei;HUANG Jian-mei;School of Chinese Materia Medica,Beijing University of Chinese Medicine;

• 1:北京中医药大学中药学院

摘要(Abstract)：

糖尿病视网膜病变(DR)和糖尿病肾病(DN)是最常见的糖尿病并发症,也是糖尿病致死致残的主要原因,临床报道显示复方血栓通胶囊对DR和DN均有较好的治疗作用,但复方血栓通胶囊中的化学成分在眼球和肾脏中的分布尚无报道,该研究主要探索复方血栓通胶囊中的化学成分在比格犬眼球和肾脏中的分布情况。比格犬连续灌胃给予复方血栓通胶囊3d后,取眼球和肾脏,运用高效液相色谱-高分辨液质联用技术LTQ-Orbitrap鉴定其中的化学成分,通过与对照品及相关文献中化学成分的精确相对分子质量、保留时间以及二级碎片信息比对,确认化合物。该研究在眼球和肾脏中分别鉴定出14,19个三七皂苷类成分。鉴定结果为阐释复方血栓通胶囊在治疗DR和DN的有效成分奠定了一定的物质基础。
Diabetic retinopathy( DR) and diabetic nephropathy( DN) are the most common complications of diabetes,and the main causes of death and disability of diabetes. Clinical reports showed that Fufang Xueshuantong capsule( FXT) had effective curative effect on DR and DN,but there was no report on the distribution of chemical compounds of FXT in beagle dog eyes and kidneys. In this study,FXT was given by gavage administration in Beagle dogs for 3 days,and then their eyeballs and kidneys were taken. The chemical compounds in beagle dog eyes and kidneys were detected by HPLC LTQ-Orbitrap technology. Furthermore,by comparing with the data from retrieving literature and references,the chemical compounds were identified by the accurate mass,retention time( tR),and MS / MS. Fourteen and 19 types of notoginsenosides were respectively identified in eyeballs and kidneys in this study,and these results could lay foundation for clarifying the effective ingredients of FXT in treatment of DR and DN.

关键词(KeyWords)： 复方血栓通胶囊;三七皂苷;眼球;肾脏;HPLC LTQ-Orbitrap
Fufang Xueshuantong capsule;notoginsenosides;eyeball;kidney;HPLC LTQ-Orbitrap

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2011ZX09201-201-22)

作者(Authors): 江美芳;王勤辉;庞欢欢;田悦;王晓烨;孙璐;高伟;黄建梅;
JIANG Mei-fang;WANG Qin-hui;PANG Huan-huan;TIAN Yue;WANG Xiao-ye;SUN Lu;GAO Wei;HUANG Jian-mei;School of Chinese Materia Medica,Beijing University of Chinese Medicine;

参考文献(References)：

• [1]程莹雪.复方血栓通胶囊治疗糖尿病视网膜病变的临床疗效观察[J].中国医药指南,2013,11(33):215.
• [2]周莎.芪明颗粒对比复方血栓通胶囊治疗非增殖期糖尿病视网膜病变的临床观察[D].武汉:湖北中医药大学,2013.
• [3]李辛,钱峰,刘利娟.复方血栓通胶囊治疗糖尿病视网膜病变的疗效观察[J].中国医药指南,2012,10(12):666.
• [4]刘杰,冯烈,卢筱华,等.复方血栓通胶囊对糖尿病肾病的疗效观察[J].今日药学,2012,22(5):289.
• [5]邢玉微,唐文佳,邹俊杰,等.复方血栓通胶囊对糖尿病大鼠肾脏的保护作用[J].第二军医大学学报,2010,31(10):1091.
• [6]王国芳,吴织芬,王勤涛.中药有效成分研究与中医现代化[J].医学与哲学,2005,26(8):59.
• [7]Liang Y,Wang G J,Xie L.Recent development in liquid chromatography/mass spectrometry and emerging technologies for metabolite identification[J].Curr Drug Metabo,2011,12(4):329.
• [8]Scigelova M,Makarov A.Advances in bioanalytical LC-MS using the Orbitrap(TM)mass analyzer[J].Bioanalysis,2009,4(1):741.
• [9]Makarov A,Scigelova M.Coupling liquid chromatography to Orbitrap mass spectrometry[J].J Chromatogr A,2010,1217(25):3938.
• [10]Jang K S,Baik J E,Kang S S.Identification of staphylococcal lipoteichoic acid-binding proteins in human serum by high-resolution LTQ-Orbitrap mass spectrometry[J].Mol Immunol,2012,3(50):177.
• [11]Caprioli G,Cahill M G,Vittori S.Liquid chromatography-hybrid linear ion trap-high-resolution mass spectrometry(LTQ-Orbitrap)method for the determination of glycoalkaloids and their aglycons in potato samples[J].Food Anal Methods,2014,7(7):1367.
• [12]Liu J H,Wang X,Cai S Q,et al.Analysis of the constituents in the Chinese drug notoginseng by liquid chromatography-electrospray mass spectrometry[J].J Chin Pharm Sci,2004,13(4):225.
• [13]徐文,丘小惠,张靖,等.超高压液相/电喷雾-LTQ-Orbitrap质谱联用技术分析三七根中皂苷类成分[J].药学学报,2012,47(6):773.
• [14]Lai C M,Li S P,Yu H,et al.A rapid HPLC-ESI-MS/MS for qualitative and quantitative analysis of saponins in"XUESETONG"injection[J].J Pharm Biomed Anal,2006,40(3):669.
• [15]王放.基于LC-HR-MSn的苦碟子及血栓通注射剂化学成分研究[D].北京:北京中医药大学,2014.