中国中药杂志

2019, v.44(17) 3608-3614

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不同产地猪苓多糖含量及猪苓多糖中的单糖组成研究
Study on polysaccharide content and monosaccharide composition of Polyporus umbellatus from different production areas

宋瑞琦;南铁贵;袁媛;金艳;杨全;张敏;胡开永;
SONG Rui-qi;NAN Tie-gui;YUAN Yuan;JIN Yan;YANG Quan;ZHANG Min;HU Kai-yong;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences;Monyan Pharma;

摘要(Abstract):

为更好地开发利用猪苓资源、研究猪苓多糖的单糖组成,该文分别采用蒽酮-硫酸法、优化的超高效液相色谱-电喷雾串联三重四极杆质谱技术(UPLC-MS/MS),对17个产地猪苓药材进行了多糖含量、单糖组分及单糖含量比较。结果表明,不同产地猪苓总多糖含量存在一定差异,总体上陕西、四川猪苓总多糖含量相对较高(1. 15%,1. 90%);猪苓多糖主要由葡萄糖、葡萄糖醛酸、半乳糖、核糖、木糖、阿拉伯糖、甘露糖及岩藻糖8种单糖组成,其中葡萄糖质量分数最高(17. 65 mg·g-1),核糖质量分数最低(0. 13 mg·g-1),部分产地猪苓样品中还检出果糖、鼠李糖及半乳糖醛酸。通过主成分聚类分析,17个产地的猪苓样品主要聚为3类,来自黑龙江五常的样品单独聚为一类。上述结果将为猪苓资源的合理利用、猪苓多糖中各单糖连接顺序及药理活性研究提供依据。
In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas. The monosaccharides were derived by 1-phenyl-3-methyl-5-pyrazolone( PMP) and the derivatives were identified by UPLC-MS/MS and the content of each monosaccharide component was determined simultaneously. The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1. 15% and 1. 90%) was relatively higher than that of others areas. Polysaccharides from P. umbellatus was mainly composed of eight monosaccharides,including glucose,glucuronic acid,galactose,ribose,xylose,arabinose,mannose and fucose. The contents of glucose( 17. 65 mg·g-1) was higher than others. The ribose was the lowest( 0. 13 mg·g-1). In addition,fructose,rhamnose and galacturonic acid were also detected in some samples. Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately. The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.

关键词(KeyWords): 猪苓多糖;单糖组成;柱前衍生化;UPLC-MS/MS
Polyporus umbellatus polysaccharide;monosaccharide composition;pre-column derivation;UPLC-MS/MS

Abstract:

Keywords:

基金项目(Foundation): 国家科技基础性工作专项(2015FY111500);; 中央级公益性科研院所基本科研业务费专项(ZZ10-008);; 中央本级重大支减项目(2060302);; 国家重点研发计划项目(2017YFC1700706)

作者(Author): 宋瑞琦;南铁贵;袁媛;金艳;杨全;张敏;胡开永;
SONG Rui-qi;NAN Tie-gui;YUAN Yuan;JIN Yan;YANG Quan;ZHANG Min;HU Kai-yong;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences;Monyan Pharma;

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