宋瑞琦;南铁贵;袁媛;金艳;杨全;张敏;胡开永;
SONG Rui-qi;NAN Tie-gui;YUAN Yuan;JIN Yan;YANG Quan;ZHANG Min;HU Kai-yong;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences;Monyan Pharma;

• 1:广东药科大学中药学院
• 2:中国中医科学院中药资源中心道地药材国家重点实验室培育基地
• 3:湖北梦阳药业

摘要(Abstract)：

为更好地开发利用猪苓资源、研究猪苓多糖的单糖组成,该文分别采用蒽酮-硫酸法、优化的超高效液相色谱-电喷雾串联三重四极杆质谱技术(UPLC-MS/MS),对17个产地猪苓药材进行了多糖含量、单糖组分及单糖含量比较。结果表明,不同产地猪苓总多糖含量存在一定差异,总体上陕西、四川猪苓总多糖含量相对较高(1. 15%,1. 90%);猪苓多糖主要由葡萄糖、葡萄糖醛酸、半乳糖、核糖、木糖、阿拉伯糖、甘露糖及岩藻糖8种单糖组成,其中葡萄糖质量分数最高(17. 65 mg·g-1),核糖质量分数最低(0. 13 mg·g-1),部分产地猪苓样品中还检出果糖、鼠李糖及半乳糖醛酸。通过主成分聚类分析,17个产地的猪苓样品主要聚为3类,来自黑龙江五常的样品单独聚为一类。上述结果将为猪苓资源的合理利用、猪苓多糖中各单糖连接顺序及药理活性研究提供依据。
In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas. The monosaccharides were derived by 1-phenyl-3-methyl-5-pyrazolone( PMP) and the derivatives were identified by UPLC-MS/MS and the content of each monosaccharide component was determined simultaneously. The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1. 15% and 1. 90%) was relatively higher than that of others areas. Polysaccharides from P. umbellatus was mainly composed of eight monosaccharides,including glucose,glucuronic acid,galactose,ribose,xylose,arabinose,mannose and fucose. The contents of glucose( 17. 65 mg·g-1) was higher than others. The ribose was the lowest( 0. 13 mg·g-1). In addition,fructose,rhamnose and galacturonic acid were also detected in some samples. Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately. The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.

关键词(KeyWords)： 猪苓多糖;单糖组成;柱前衍生化;UPLC-MS/MS
Polyporus umbellatus polysaccharide;monosaccharide composition;pre-column derivation;UPLC-MS/MS

Abstract:

Keywords:

基金项目(Foundation): 国家科技基础性工作专项(2015FY111500);; 中央级公益性科研院所基本科研业务费专项(ZZ10-008);; 中央本级重大支减项目(2060302);; 国家重点研发计划项目(2017YFC1700706)

作者(Author): 宋瑞琦;南铁贵;袁媛;金艳;杨全;张敏;胡开永;
SONG Rui-qi;NAN Tie-gui;YUAN Yuan;JIN Yan;YANG Quan;ZHANG Min;HU Kai-yong;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences;Monyan Pharma;

Email:

DOI:

参考文献(References)：

• [1]中国药典.一部[S]. 2015.
• [2]王天媛,张飞飞,任跃英,等.猪苓化学成分及药理作用研究进展[J].上海中医药杂志,2017,51(4):109.
• [3] Li H L,Zhuan Y,Xiong Q P,et al. Renoprotective effect and mechanism of polysaccharide from Polyporus umbellatus sclerotia on renal fibrosis[J]. Carbohydrate Polym,2019,212(15):1.
• [4]江泽波,胡金萍,温晓文,等.猪苓多糖对巨噬细胞RAW264. 7的双向免疫调节作用[J].免疫学杂志,2014,30(12):1033.
• [5] Dai H,Han X Q,Gong F Y,et al. Structure elucidation and immunological function analysis of a novel D-glucan from the fruit bodies of Polyporus umbellatus(Pers.)Fries[J]. Glycobiology,2012,22(12):1673.
• [6]陶娟,李涛,薛国平,等.猪苓多糖在血液透析患者乙肝疫苗免疫接种中的应用研究[J].中国医药指南,2013,11(22):7.
• [7] Bao S H,Zha X Q,Hao J. In vitro antioxidant activity of polysaccharides with different molecular weights from Dendrobium candidum[J]. Food Sci,2009,30(21):123.
• [8]芦红云,吴天祥,汤庆莉,等.天麻醇提物对灰树花胞外多糖单糖组成和生物活性的影响[J].食品科学技术学报,2018,36(3):40.
• [9]柏慧,张仁慈,王曦烨,等.三重四级杆质谱技术在中药研究中的应用进展[J].内蒙古民族大学学报:自然科学版,2018,33(3):208.
• [10]梁宇庭,周骏辉,南铁贵,等.柱前衍生化UPLC-MS/MS测定12种单糖含量的方法学研究及其应用[J].中国中药杂志,2018,43(22):4469.
• [11]龚力民,刘伟,卓海燕,等.五倍子多糖相对分子质量和单糖组成的测定[J].中国实验方剂学杂志,2015,21(24):34.
• [12]宝炉丹.糖类与类糖物质高效液相色谱衍生化方法研究[D].沈阳:沈阳药科大学,2007.
• [13]郭宁,武泽宇,王建刚,等.不同产地、生长年限、采收期猪苓中三种有效成分的含量分析[J].特产研究,2019,41(1):72.
• [14]夏琴,李敏,周进,等.不同产地、商品规格及生长年限猪苓麦角甾醇及多糖的含量分析[J].中药材,2015,38(1):45.
• [15]杨海燕,殷莉丽,曾晖,等.海拔、坡向和年限与猪苓产量、质量相关性研究[J].中药与临床,2017,8(2):8.
• [16]郭宁,乐智勇,王弯弯,等.不同来源、不同等级猪苓饮片中总多糖的含量测定[J].中国实验方剂学杂志,2019,25(5):156.
• [17]何鹏飞.猪苓菌核多糖的分离纯化、结构鉴定和溶液构象研究[D].杭州:浙江工业大学,2016.