中国中药杂志

2015, v.40(08) 1543-1547

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狭基线纹香茶菜水溶性总黄酮中6种碳苷黄酮的含量测定和抗肿瘤活性研究
Determination of six C-Glycoside flavones and antitumor activity of water-soluble total flavonoids from Isodon lophanthoides var.gerardianus

张扬;唐海明;黎爱;徐兰芳;陈建南;黄松;贺莲;
ZHANG Yang;TANG Hai-ming;LI Ai;XU Lan-fang;CHEN Jian-nan;HUANG Song;HE Lian;New Drug Research & Development Center,Guangzhou University of Chinese Medicine;Xinhua College of Sun Yat-Sen University;

摘要(Abstract):

该文建立了HPLC同时测定狭基线纹香茶菜水溶性总黄酮中6种碳苷黄酮含量的方法,并对狭基线纹香茶菜水溶性总黄酮进行抗肿瘤活性评价。采用Kromasil 100-5 C_(18)(4.6 mm×250 mm,5μm)色谱柱;流动相为甲醇-乙腈-0.5%甲酸梯度洗脱;体积流量0.8 mL·min~(-1),检测波长334 nm;柱温为室温(25℃)。以HepG2细胞为供试细胞株,采用MTT法评价水溶性总黄酮的抗肿瘤活性。新西兰牡荆苷2、新西兰牡荆苷3、异夏佛塔苷、夏佛塔苷、牡荆苷和芹菜素-6,8-二-C-α-L-吡喃阿拉伯糖苷线性范围分别为0.25~2.53,0.12~1.20,0.37~3.69,0.16~1.63,0.19~1.92,0.14~1.42μg;平均回收率(n=6)分别为99.6%,100.2%,99.6%,97.9%,98.8%,98.6%,RSD分别为0.87%,2.0%,1.8%,1.5%,1.2%,1.2%。以IC_(50)值作为评价指标,测得狭基线纹香共菜水溶性总黄酮在给药24,48,72 h后抑制HepG2细胞增殖的IC_(50)值分别为1.89,1.71,1.51g·L~(-1)。本法快速、简便、准确,重复性好,可用于狭基线纹香共菜水溶性总黄酮的质量控制。狭基线纹香茶菜水溶性总黄酮对HepG2细胞增殖有一定的抑制作用。
This research established an HPLC method for determination of six C-Glycoside flavones of warer-soluble total flavonoids from Isodon lophanthoides var.gerardianus(Benth.)H.Hara,and studied the antitumor activity of the warer-soluble total flavonoids.The HPLC system consisted of Kromasil 100-5 C_(18)(4.6 mm ×250 mm,5 μm)column and a solution system of methanol,acetonitrile and 0.5% formic acid gradient elution at a flow rate of 0.8 mL·min~(-1) and the wavelength of detector was at 334 nm.The column temperature was 25 °C.The antitumor activity of water-soluble flavonoids was assayed using HepG2 cell as the tested cell.The linear ranges of viceninⅡ,vicenin Ⅲ,isoschaftoside,schaftoside,vitexin,6,8-di-C-a-L-arabinosylapigenin were 0.25-2.53,0.12-1.20,0.37-3.69,0.16-1.63,0.19-1.92,0.14-1.42 μg,respectively.The average recoveries(n = 6) were 99.6%(RSD 0.87%),100.2%(RSD 2.0%),99.6%(RSD 1.8%),97.9%(RSD 1.5%),98.8%(RSD 1.2%),98.6%(RSD 1.2%),respectively.After exposure in 24,48,72 h,the total flavonoids showed inhibitory effect on the proliferation of HepG2 cells with IC_(50) as the evaluation index,the IC_(50) values of 1.89,1.71,1.51 g·L~(-1),respectively.The method is quick,simple and accurate with good reproducibility,and can be used for determination of viceninⅡ,viceninⅢ,isoschaftoside,schaftoside,vitexin,6,8-di-C-α-L-arabinosylapigenin in the warer-soluble total flavonoids from I.lopharuhoides var.gerardianus.The warer-soluble total flavonoids from I.lopharuhoides have inhibitory effect on the proliferation of HepG2 cells.

关键词(KeyWords): 狭基线纹香茶菜;水溶性黄酮;高效液相色谱法;HepG2
Isodon lophanthoides;warer-soluble flavonoids;HPLC;HepG2

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑计划项目(2012BAI29B09)

作者(Author): 张扬;唐海明;黎爱;徐兰芳;陈建南;黄松;贺莲;
ZHANG Yang;TANG Hai-ming;LI Ai;XU Lan-fang;CHEN Jian-nan;HUANG Song;HE Lian;New Drug Research & Development Center,Guangzhou University of Chinese Medicine;Xinhua College of Sun Yat-Sen University;

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