濮春娟;李鹏英;罗钰枝;周修腾;邵爱娟;陈美兰;
PU Chun-juan;LI Peng-ying;LUO Yu-zhi;ZHOU Xiu-teng;SHAO Ai-juan;CHEN Mei-lan;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;China National Traditional Chinese Medicine Corporation;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;

• 1:中国中医科学院中药资源中心道地药材国家重点实验室培育基地
• 2:中国中药有限公司
• 3:中国中医科学院中药研究所

摘要(Abstract)：

丛枝菌根真菌与栽培丹参形成共生体后产生诸多有益的作用,如提高药材产量、促进有效成分积累,减少病害的发生等,然而施用杀菌剂是否会影响菌根效益,目前相关研究甚少。基于此,该研究以丛枝菌根真菌的侵染强度、丹参生长以及有效成分含量作为评价指标,研究4种不同类型的杀菌剂(多菌灵、多抗霉素、甲基拖布津、代森锰锌)对菌根效益的影响。实验结果表明不同种类的杀菌剂作用不同。施用代森锰锌对丹参菌根效益的抑制作用最强,代森锰锌处理会显著降低菌根侵染率和侵染强度,显著抑制丛枝菌根真菌对丹参生长以及有效成分积累的促进作用。多抗霉素处理对菌根侵染率和侵染强度无显著影响,反而在促进丹参地上部分生长以及迷迭香酸的积累等方面与菌根具有协同作用。4种不同类型的杀菌剂对丛枝菌根侵染及菌根效益的抑制作用大小为代森锰锌>甲基托布津、多菌灵>多抗霉素。因此,在进行丹参菌根化栽培时可以配合使用多抗霉素等生物杀菌剂,避免使用代森锰锌等化学杀菌剂进行病害防治。
Arbuscular mycorrhizal fungi provided is beneficial to Salvia miltiorrhiza for increasing yield, promoting the accumulation of active ingredients, and alleviating S. miltiorrhiza disease etc. However, the application of fungicides will affect the benefit of arbuscular mycorrhizal fungi and there is little research about it. This article study the effect of four different fungicides: carbendazim, polyoxin, methyl mopazine, and mancozeb on mycorrhiza benefit to S. miltiorrhiza by the infection intensity of arbuscular mycorrhizal fungi, the growth of S. miltiorrhiza, and the content of active ingredients. Results showed that different fungicides had different effects. The application of mancozeb had the strongest inhibitory effect on the mycorrhizal benefit to S. miltiorrhiza. Mancozeb significantly reduced the mycorrhizal colonization and the beneficial effect of arbuscular mycorrhizal fungi on the growth and the accumulation of active components of S. miltiorrhiza. The application of polyoxin had no significant effect on mycorrhizal colonization. Instead, it had a synergistic effect with the mycorrhizal benefit to promoting the growth and accumulation of rosmarinic acid of S. miltiorrhiza. The inhibitory strengths of four fungicides are: mancozeb>thiophanate methyl, carbendazim>polyoxin. Therefore, we recommend applying biological fungicides polyoxin and avoid applying chemical fungicides mancozeb for disease control during mycorrhizal cultivation of S. miltiorrhiza.

关键词(KeyWords)： 丹参;丛枝菌根真菌;杀菌剂;菌根效益
Salvia miltiorrhiza;arbuscular mycorrhizal fungi;fungicide;mycorrhizal effect

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(81773849,81803658);; 中央级公益性科研院所基本科研业务费专项(ZZ13-YQ-088,ZZXT201803)

作者(Author): 濮春娟;李鹏英;罗钰枝;周修腾;邵爱娟;陈美兰;
PU Chun-juan;LI Peng-ying;LUO Yu-zhi;ZHOU Xiu-teng;SHAO Ai-juan;CHEN Mei-lan;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;China National Traditional Chinese Medicine Corporation;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;

Email:

DOI: 10.19540/j.cnki.cjcmm.20201222.101

参考文献(References)：

• [1] 国家药典委员会.中华人民共和国药典.一部[S].北京：中国医药科技出版社，2015:76.
• [2] 李巧玉，刘杨，包华音.近5年丹参化学成分及药理作用研究进展[J].食品与药品，2014,16(2):145.
• [3] 陈芬燕，郭韧，张毕奎.丹参酮ⅡA的心血管药理作用研究进展[J].中国中药杂志，2015,40(9):1649.
• [4] 王铁霖，关巍，孙楷，等.丹参常见病害的病原、发病规律及综合防治[J].中国中药杂志，2018,43(11):2402.
• [5] 杨立，缪作清，杨光，等.丹参枯萎病及其病原菌的研究[J].中国中药杂志，2013,38(23):4040.
• [6] 杨银慧，豆小文，孔维军，等.我国中药材中农药登记现状及污染分析[J].中国中药杂志，2013,38(24):4238.
• [7] 王希付，张雪，赵荣华，等.丛枝菌根真菌在药用植物中的作用及机制研究进展[J].中国实验方剂学杂志，2020,26(11):217.
• [8] 王雪，陈美兰，杨光，等.丛枝菌根真菌与哈茨木霉菌合用对连作丹参生长及质量的影响[J].中国中药杂志，2014,39(9):1574.
• [9] CHEN M L,YANG G,LIU D H,et al.Inoculation with Glomus mosseae improves the growth and salvianolic acid B accumulation of continuously cropped Salvia miltiorrhiza [J].Appl Sci,2017,7:692.
• [10] 赵玲，滕应，骆永明.中国农田土壤农药污染现状和防控对策[J].土壤，2017,49(3):417.
• [11] KUMAR A,TEWARI R,SHUKLA A.Effect of some fungicides on development of vesicular arbuscular mycorrhizae and growth of Sesbania sesban [J].Indian J Agrofor,2005,7(2):55.
• [12] YADAV A,AGGARWAL A.Effect of benomyl and dithane M-45 on mycoflora,mycorrhizae and growth enhancement of He-lianthus annuus L.[J].N Y Sci J,2014,7(11):7.
• [13] PHILLIPS J M,HAYMAN D S.Improved procedures for clearing and ataining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection [J].Tran Br Mycol Soc,1970,55:158.
• [14] 李鹏英，杨光，周修腾，等.栽培基质酸碱度对丹参菌根效益的影响[J].中国中药杂志，2018,43(17):3460.
• [15] RABAB A M,REDA E A.Impact of Ridomil,Bavistin and Agrothoate on arbuscular mycorrhizal fungal colonization,biochemical changes and potassium content of cucumber plants[J].Ecotoxicology,2019,doi:10.1007/s10646-019-02042-0.
• [16] MALLMANN G C,SOUSA J P,SUNDH I,et al.Placing arbuscular mycorrhizal fungi on the risk assessment test battery of plant protection products (PPPs) [J].Ecotoxicology,2018,27:809.
• [17] KOIDE R T,MOSSE B.A history of research on arbuscular mycorrhizal [J].Mycorrhiza,2004,14:145.
• [18] JIN H,GERMIDA J J,WALLEY F L.Suppressive effects of seed-applied fungicides on arbuscular mycorrhizal fungi (AMF) differ with fungicide mode of action and AMF species [J].Appl Soil Ecol,2013,72:22.
• [19] 陈武英.代森锰锌及其代谢物乙撑硫脉在苹果和土壤中的动态研究[D].长沙：湖南农业大学，2010.
• [20] 雷永和，李天飞.烟用化肥与农药[M].昆明：云南科技出版社，1998:168.
• [21] 吴家全，李军民.多抗霉素研究现状与市场前景[J].农药科学与管理，2010(3):15.