中国中药杂志

2011, v.36(03) 330-333

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不同种源高良姜遗传多样性的AFLP分析
AFLP analysis of genetic diversity of Alpinia officinarum

杨全;张春荣;陈虎彪;滕希峰;唐晓敏;
YANG Quan1,ZHANG Chunrong1,CHEN Hubiao2,TENG Xifeng1,TANG Xiaomin1(1.School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;2.School of Chinese Medicine,Hong Kong Baptist University,Hong Kong,China)

摘要(Abstract):

目的:研究不同种源高良姜的遗传多样性,探求各种质间的亲缘关系,为合理利用高良姜种质资源及优良品种选育奠定理论基础。方法:采用扩增片段长度多态性(AFLP)技术,对8个种源地的高良姜进行遗传多态性分析,将扩增出的条带作为原始矩阵,用NTSYSpc-2.11F软件计算并分析高良姜种质间的相似度,构建遗传系统进化树。结果:通过筛选得到8对AFLP引物组合,共扩增出1 120个DNA条带,多态性条带为1 044个,多态性位点平均为92.57%;通过构建系统进化树,将8个种源地的高良姜种质分为3类。结论:高良姜种质间存在较高多态性,遗传多样性丰富。
Objective: To explore the genetic diversity and relationship of different Alpinia officinarum germplasm.Method: Amplified fragment length polymorphism(AFLP) markers were developed to analyze genetic polymorphism in A.officinarum from eight resources.The amplified fragments were used as primary matrix with NTSYSpc-2.11F software to analyze the similarity between the A.officinarum germplasm and to construct the genetic phylogenetic tree.Result: A total of 1 120 fragments were genotyped using AFLP with eight prime combinations.Analysis identified 1 044 polymorphic fragments,accounting for 92.57% of the total detected variation.Genetic phylogenetic tree analysis indicates that three categories can be divided among the eight resources of A.officinarum.Conclusion: Significant polymorphism and genetic diversity can be observed among A.officinarum germplasm resources.

关键词(KeyWords): 高良姜;种质资源;AFLP;遗传多样性
Alpinia officinarum;germplasm resources;amplified fragment length polymorphism(AFLP);genetic diversity

Abstract:

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基金项目(Foundation): 国家科技基础条件平台工作项目(2005DKA21000);; 国家科技基础性工作专项重点项目(2007FY110600);; 广东省科技计划项目(2009B060700076);; 广东药学院博士启动项目;广东药学院重点培养青年教师项目

作者(Author): 杨全;张春荣;陈虎彪;滕希峰;唐晓敏;
YANG Quan1,ZHANG Chunrong1,CHEN Hubiao2,TENG Xifeng1,TANG Xiaomin1(1.School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;2.School of Chinese Medicine,Hong Kong Baptist University,Hong Kong,China)

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