中国中药杂志

2017, v.42(07) 1292-1299

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

忍冬叶活性成分周期变化及其代谢关系研究
Research on periodical changes and metabolism relations of active components in Lonicera japonica leaves

刘伟;杨帆;刘峰;周冰谦;耿岩玲;李圣波;郭兰萍;王晓;
LIU Wei;YANG Fan;LIU Feng;ZHOU Bing-qian;GENG Yan-ling;LI Sheng-bo;GUO Lan-ping;WANG Xiao;Key Laboratory of Traditional Chinese Medicine Quality Control Technology,Shandong Analysis and Test Center;Shandong Yate Ecological Technology Co.,Ltd.;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

该文通过对忍冬叶初生及次生代谢过程进行研究,探讨不同发育阶段忍冬叶代谢规律。在每月下旬采收金银花上部5~8片完全展开叶,采用HPLC测定相关代谢指标。结果表明,忍冬叶含水量在3月份最高,达到78.59%,12月份最低,为60.83%;总糖量在冬季最高,1月份达到最高275.8 mg·g~(-1),其他时间明显降低;忍冬叶中的绿原酸、新绿原酸、木犀草苷和咖啡酸的含量变化基本一致,四者的含量均在3月达到最高,分别为42.79,2.01,7.13,0.16 mg·g~(-1)。忍冬叶的初生代谢产物和次生代谢产物含量在3—5月达到较高水平,与有效成分含量相关的因素主要有钾、镁、磷、门冬氨酸、苏氨酸、脯氨酸、缬氨酸、胱氨酸、异亮氨酸和苯丙氨酸。
The study is aimed to explore the metabolism rule of Lonicera japonica by investigating the primary and secondary metabolism process in different growth periods.HPLC and other methods were used to measure metabolism indexes of leaves collected in last ten days of every month.The results suggested that the maximum(78.59%) and minimum(60.83%) of water content were found in March and December.The content of total sugar reached a high level from December to February and the maximum(275.8 mg·g~(-1))appeared in October,while it reduced significantly at other time.The change of chlorogenic acid,neochlorogenic acid,galuteolin,caffeic acid were basically consistent and the highest content of them synchronously appeared(42.79,2.01,7.13,0.16 mg·g~(-1)) in March.The content of primary and secondary metabolite in L.japonica leaves reached a high level from March to May,and the main related elements with effective components were K,Mg,P,aspartate,threonine,proline,valine,cysteine,isoleucine and phenylalanine.

关键词(KeyWords): 忍冬叶;活性成分;代谢关系
Lonicera japonica leaves;active components;metabolism relations

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(81473298);; 山东省攻关计划项目(2014GSF119031,2015GSF119021);; 山东省三院联合基金项目(ZR2016YL006);; 山东省泰山学者岗位专家支持项目;; 山东省科学院先导项目

作者(Author): 刘伟;杨帆;刘峰;周冰谦;耿岩玲;李圣波;郭兰萍;王晓;
LIU Wei;YANG Fan;LIU Feng;ZHOU Bing-qian;GENG Yan-ling;LI Sheng-bo;GUO Lan-ping;WANG Xiao;Key Laboratory of Traditional Chinese Medicine Quality Control Technology,Shandong Analysis and Test Center;Shandong Yate Ecological Technology Co.,Ltd.;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

Email:

DOI: 10.19540/j.cnki.cjcmm.20170222.013

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享