中国中药杂志

2013, v.38(11) 1653-1656

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丹参红花混煎液中丹参素、羟基红花黄色素A、迷迭香酸、紫草酸和丹酚酸B的HPLC测定方法
Simultaneous determination of danshensu,hydroxysafflor yellow A,rosmarinic acid,lithospermic acid,salvianolic acid B in water extract of mixed Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos by HPLC

厉瑶;郭正泰;龚行楚;瞿海斌;
LI Yao,GUO Zheng-tai,GONG Xing-chu,QU Hai-bin(Pharmaceutical Informatics Institute,Zhejiang University,Hangzhou 310058,China)

摘要(Abstract):

目的:建立同时测定丹参红花混煎液中丹参素、羟基红花黄色素A、迷迭香酸、紫草酸和丹酚酸B含量的HPLC法。方法:采用梯度洗脱高效液相色谱法,色谱柱为ZORBAX Eclipse Plus C18柱(4.6 mm×100 mm,1.8μm);以甲酸-500 mmol.L-1甲酸铵-水(0.5∶10∶90)为流动相A,0.5%甲酸-乙腈为流动相B;检测波长280 nm(丹参素、迷迭香酸、紫草酸、丹酚酸B)和380 nm(羟基红花黄色素A);流速0.5 mL.min-1;柱温30℃。结果:建立了同时测定丹参红花混煎液中5种成分含量的方法。丹参素、羟基红花黄色素A、迷迭香酸、紫草酸和丹酚酸B质量浓度分别在3.51~84.2,3.48~83.5,1.90~45.6,2.47~59.3,37.9~909.6 mg.L-1与峰面积积分值线性关系良好(R2>0.999 3),加样回收率分别为99.1%,102%,102%,98.5%,101%。建立的HPLC具有良好的精密度、重复性和稳定性。结论:该方法操作简便,精密度好,结果准确可靠。
Objective: To develop an HPLC method to determine the contents of danshensu,hydroxysafflor yellow A,rosmarinic acid,lithospermic acid,salvianolic acid B in the water extract of mixed Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos simultaneously.Method: The separation were carried out at 30 ℃ on a ZORBAX Eclipse Plus C18 column(4.6 mm×100 mm,1.8 μm) with formic acid-500 mmol·L-1 ammonium formate-water solution(0.5∶10∶90) as mobile phase A and acetonitrile-formic acid solution(100∶0.5) as mobile phase B in gradient mode at a flow rate of 0.5 mL·min-1.Detection wavelengths were 280 nm for danshensu,rosmarinic acid,lithospermic acid,salvianolic acid B,and 380 nm for hydroxysafflor yellow A.Result: The 5 components were separated well with a good linearity(R2>0.999 3) in the range of the test concentration.The average recoveries of danshensu,hydroxysafflor yellow A,rosmarinic acid,lithospermic acid,and salvianolic acid B were 99.1%,102%,102%,98.5% and 101%,respectively.Conclusion: This method is simple,accurate,and repeatable.

关键词(KeyWords): 丹参;红花;丹红注射液;高效液相色谱法;中药质量控制
Salviae Miltiorrhizae Radix et Rhizoma;Carthami Flos;Danhong injection;HPLC;quality control of traditional Chinese medicine

Abstract:

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基金项目(Foundation): 浙江省自然科学基金项目(LQ12H29004)

作者(Author): 厉瑶;郭正泰;龚行楚;瞿海斌;
LI Yao,GUO Zheng-tai,GONG Xing-chu,QU Hai-bin(Pharmaceutical Informatics Institute,Zhejiang University,Hangzhou 310058,China)

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