白海波,王剑飞,龙娟
BAI Hai-bo 1, WANG Jian-fei 2, LONG Juan 2 (1.Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China; 2.H ainan Yangshengtang Medical Research Center, Hangzhou 310007,China)

• 1:浙江中医学院
• 2:海南养生堂药物研究中心
• 3:海南养生堂药物研究中心 浙江杭州310053
• 4:浙江杭州310007

摘要(Abstract)：

目的 :分别以何首乌提取物 2 ,3 ,4,5 四羟基二苯乙烯 2 O β D 葡萄糖苷水平和提取物抗氧化能力为检测指标对何首乌进行提取工艺设计 ,并检验 2 ,3 ,4,5 四羟基二苯乙烯 2 O β D 葡萄糖苷和抗氧化能力之间的相关性。方法 :采用正交实验法以乙醇浓度 (A)、溶剂量 (B)、提取时间 (C)和提取次数 (D) 4个因素 ,每个因素选取 3个水平进行实验。结果 :因素A ,D对 2 ,3 ,4,5 四羟基二苯乙烯 2 O β D 葡萄糖苷的含量有显著影响 ,同样对何首乌的抗氧化能力影响也最为显著 ,经检验 2 ,3 ,4,5 四羟基二苯乙烯 2 O β D 葡萄糖苷含量与何首乌的抗氧化能力之间呈显著线性相关 (P <0 .0 1)。结论 :以 60 %乙醇 ,8倍量提取 3次 ,每次 1.5h为最佳提取工艺。
Objective:To optimize the extraction proc ess of root of Polygoni multif lori and validate the interrelation between antioxdative capacity and the co ntent of 2,3,4,5-tetrahydroxystilbene-2-O-β-D-glucoside in root of P. multiflori. Method: The optimum extraction was abserved with the orthogonal design; 2,3,4,5-tetrahydroxystilbene-2-O-β-D-glucoside was determined by HPL C and the antioxidative capacity by using photochemiluminescence detection method with the Photochem supplied by Analytik Jena AG; the concentration of ethanol , amount of ethanol, extraction time and extraction times were the four factors in the e xtraction. Result: The concentration of ethanol and extrac tion times had signif icant effect on the content of 2,3,4,5-tetrahydroxystilbene-2-O-β-D-g lucoside a nd the antioxidative capacity of the crude extract. Conclusion: The best extract ion process is to extract three times by using 10 fold EtOH(60%),refluxing at 85 ℃ and to extracte one and half hours each time.

关键词(KeyWords)： 何首乌;2,3,4,5-四羟基二苯乙烯-2-O-β-D葡萄糖苷;提取工艺;抗氧化
Polygonum multiflorum; 2,3,4,5-te trahydroxystilbene-2-O-β-D-glucoside; extraction process; antioxidative capacity

Abstract:

Keywords:

基金项目(Foundation): 浙江省科技厅资助项目 ( 0 2 1 1 0 3553)

作者(Author): 白海波,王剑飞,龙娟
BAI Hai-bo 1, WANG Jian-fei 2, LONG Juan 2 (1.Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China; 2.H ainan Yangshengtang Medical Research Center, Hangzhou 310007,China)

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DOI:

参考文献(References)：

• [1]　李玉芳,和玄华.何首乌现代研究进展.中成药,1997,19(5):37.
• [2]　鲁　静,粟晓黎,董海荣,等.高效液相色谱法测定何首乌及制首乌中羟芪衍生物的含量.药物分析杂志,2000,20(2):104.
• [3]　PopovG .Lewinantioxidativehomeostasis:characterizationbymeansofchemoluminescenttechnique.In:methodsinenzymologyvolume300,oxidantsandantioxidantspartBed.LesterPackerAcademicPress,1999.96.
• [4]　戚爱棣.何首乌中二苯乙烯苷提取工艺优选及炮制对其含量的影响.中草药,2002,33(7):609.
• [5]　韩志芬,顾文聪.不同何首乌提取液对老年大鼠心、肝、脑自由基代谢及脑内MAO B的影响.中国中医药科技,1995,2(1):36.
• [6]　马长华,王金星.何首乌炮制前后二苯乙烯苷含量比较.中药材,1995,18(7):350