鲍天冬;张英丰;李玉洁;杨庆;翁小刚;董宇;朱晓新;
BAO Tiandong, YANG Qing, ZHANG Yingfeng, DONG Yu, LI Yujie, ZHU Xiaoxin ( 1.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;2.Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China;3.Guang′anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China)

• 1:中国中医科学院中药研究所
• 2:广州中医药大学
• 3:中国中医科学院广安门医院

摘要(Abstract)：

目的:建立LC-MS测定大鼠血浆中芍药苷含量的方法,并探讨其在大鼠体内的药动学行为。方法:大鼠灌胃不同剂量的炒白芍提取物后不同时间点采血,LC-MS法测定血药浓度,拟合药动学模型并计算相关药动学参数。结果:芍药苷在2.5～500μg·L-1(r=9994)线性关系良好。方法学考察均符合要求。大鼠灌胃炒白芍提取物0.2,0.4,0.8g·kg-1后,半衰期基本一致,AUC与给药剂量之间呈现良好的线性关系,属于线性动力学过程。结论:该法专属性强,灵敏度高,可用于芍药苷的体内定量分析,芍药苷在大鼠体内动力学过程符合一级吸收二室模型。
Objective:To develop a LC-MS method to determine paeoniflorin concentration in rats plasma. The method was applied to investigate pharmacokinetics of paeoniflorin in rats in vivo.Method: Blood samples were collected at different time after oral administration of Radix Paeoniae Alba extract at doses of 0.2, 0.4, 0.8 g·kg-1. The paeoniflorin concentration in plasma was determined by LC-MS method. Pharmacokinetic parameters were fitted by WinNonlin 5.1 software package. Result: The linear range and the average recovery of paeoniflorin were 2.5-500 μg·L-1 (r=999 4). and more than 80%(n=5), respectively. The inner-and inter-days precision were both less than 15%. The T1/2 was similar. The relationship between dose and AUC showed good linearity. Conclusion: The method described in this report has high sensitivity and selectivity, and was suitable for pharmacokinetic study of paeoniflorin. The kinetic process of paeoniflorin in palsma showed two-compartment model after oral administration of Radix Paeoniae Alba extract at doses of 0.2, 0.4, 0.8 g·kg-1 to rats.

关键词(KeyWords)： LC-MS;炒白芍提取物;芍药苷;药代动力学
LC-MS; Radix Paeoniae Alba extract; paeoniflorin; pharmacokinetics

Abstract:

Keywords:

基金项目(Foundation): 国家科学自然基金重点项目(30930114);; 国家自然科学基金项目(90709017);; 国家“十一五”科技支撑计划项目(2006BAI08B04-4)

作者(Authors): 鲍天冬;张英丰;李玉洁;杨庆;翁小刚;董宇;朱晓新;
BAO Tiandong, YANG Qing, ZHANG Yingfeng, DONG Yu, LI Yujie, ZHU Xiaoxin ( 1.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;2.Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China;3.Guang′anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China)

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