中国中药杂志

2012, v.37(06) 803-805

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HPLC同时测定四逆汤中4种指标性成分的含量
Simultaneous determination of four components in Sini Tang by HPLC

刘晓;范林乾;蔡皓;蔡宝昌;
LIU Xiao1,2,FAN Linqian1,2,CAI Hao1,2,CAI Baochang1,2,3(1.College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210046,China; 2.Jiangsu Key Laboratory of Chinese Medicine Processing,Nanjing University of Chinese Medicine,Nanjing 210029,China; 3.Nanjing Haichang Chinese Medicine Group Corporation,Nanjing 210061,China)

摘要(Abstract):

目的:建立四逆汤中苯甲酰新乌头原碱、甘草苷、甘草酸和6-姜酚4种成分的高效液相色谱含量测定方法。方法:采用Thermo Hypersil BDS色谱柱(4.6 mm×250 mm,5μm),梯度洗脱,流速1.0 mL.min-1,检测波长235 nm。结果:苯甲酰新乌头原碱、甘草苷、甘草酸、6-姜酚均达到良好分离,线性范围分别为0.006~0.12(r=0.999 9),0.021~0.42(r=0.999 7),0.012~0.24(r=0.999 4),0.018~0.36 g.L-1(r=0.999 5);平均回收率分别为99.3%(RSD 1.5%),96.9%(RSD0.60%),100%(RSD 1.3%),100%(RSD 2.1%)。结论:该方法简便、准确、实用性强,可用于四逆汤中4种指标性成分的含量测定。
Objective: To develop an HPLC method for determining four components in Sini Tan,benzoylmesaconine,liquiritin,glycyrrhizic acid and 6-gingerol.Method: The Hypersil BDS column was adopted with gradient elution program at a flow rate of 1.0 mL·min-1 and the detection wavelength of 235 nm.Result: Benzoylmesaconine,liquiritin,glycyrrhizic acid and 6-gingerol showed good separation,with the linear range of 0.006-0.12,0.021-0.42,0.012-0.24 and 0.018-0.36 g·L-1,respectively.Their average recoveries were 99.3%,96.9%,100% and 100%,respectively;and RSD of the above four components were 1.5%,0.6%,1.3% and 2.1%,respectively.Conclusion: The method is proved to be so easy and accurate and practical that it can be used to determine the four components in Sini Tang.

关键词(KeyWords): 四逆汤;苯甲酰新乌头原碱;甘草苷;甘草酸;6-姜酚;高效液相色谱法
Sini Tang;benzoylmesaconine;liquiritin;glycyrrhizic acid;6-gingerol;HPLC

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基金项目(Foundation): 国家中医药管理局中医药行业科研专项(201007010)

作者(Author): 刘晓;范林乾;蔡皓;蔡宝昌;
LIU Xiao1,2,FAN Linqian1,2,CAI Hao1,2,CAI Baochang1,2,3(1.College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210046,China; 2.Jiangsu Key Laboratory of Chinese Medicine Processing,Nanjing University of Chinese Medicine,Nanjing 210029,China; 3.Nanjing Haichang Chinese Medicine Group Corporation,Nanjing 210061,China)

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