中国中药杂志

2019, v.44(02) 332-337

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北柴胡、竹叶柴胡、藏柴胡与小叶黑柴胡化学成分比较研究
Comparative analysis on chemical constituents in Bupleurum chinense,B. marginatum,B. marginatum var. stenophyllum and B. smithii var. parvifolium

杨印军;郑伟;郭佳琪;方伟;马百平;郭宝林;彭云霞;张东佳;
YANG Yin-jun;ZHENG Wei;GUO Jia-qi;FANG Wei;MA Bai-ping;GUO Bao-lin;PENG Yun-xia;ZHANG Dong-jia;Key Laboratory of Bioactive Substance and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College;Institute of Radiation Medicine,Academy of Military Medical Sciences;Institute of Chinese Herbal Medicines,Gansu Academy of Agricultural Sciences;

摘要(Abstract):

利用UPLC-Q-TOF-MS方法比较了北柴胡Bupleurum chinense、竹叶柴胡B. marginatum、藏柴胡B. marginatum var.stenophyllum与小叶黑柴胡B. smithii var. parvifolium之间化学成分构成,色谱分离采用Acquity HSS T3 C_(18)色谱柱(Waters,2. 1mm×100 mm,1. 8μm),0. 1%甲酸水溶液-乙腈为流动相进行梯度洗脱,质谱分析利用四极杆串联飞行时间质谱(Q-TOFMSE),通过精确相对分子质量和碎片离子结构鉴定了其中25个主要成分;而北柴胡和竹叶柴胡非常相似。采用主成分分析法(PCA)对数据进行处理,PCA结果显示该4种柴胡明显聚成3类,其中北柴胡和竹叶柴胡聚为一类,而藏柴胡、小叶黑柴胡与北柴胡之间存在明显的差异.
UPLC-Q-TOF-MS was used to analyze the chemical differences in Bupleurum. chinense,B. marginatum,B. marginatum var. stenophyllum and B. smithii var. parvifolium. Chromatographic separation was carried out on an Acquity HSS T3 C_(18) column( 2. 1 mm ×100 mm,1. 8 μm,Waters) with the mobile phase composed of 0. 1% formic acid in water-acetonitrile in the gradient elution. A hybrid quadrupole time-of-flight tandem mass spectrometry( Q-TOF-MSE) was used for mass spectrometric analysis. Finally,25 peaks were identified based on their exact mass data and fragmentation characteristics. B.chinense,B.marginatum,B. marginatum var. stenophyllum and B. smithii var. parvifolium were obviously clustered into 3 types through processing by principal component analysis( PCA). There was almost no difference between B. chinense and B. marginatum. However,the compounds existed in B. chinense were different from those in B. marginatum var. stenophyllum,and B. smithii var. parvifolium.

关键词(KeyWords): 柴胡;超高效液相色谱-四极杆串联飞行时间质谱;主要成分鉴定;主成分分析法
Bupleurum;UPLC-Q-TOF-MS~E;component identification;PCA

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 杨印军;郑伟;郭佳琪;方伟;马百平;郭宝林;彭云霞;张东佳;
YANG Yin-jun;ZHENG Wei;GUO Jia-qi;FANG Wei;MA Bai-ping;GUO Bao-lin;PENG Yun-xia;ZHANG Dong-jia;Key Laboratory of Bioactive Substance and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College;Institute of Radiation Medicine,Academy of Military Medical Sciences;Institute of Chinese Herbal Medicines,Gansu Academy of Agricultural Sciences;

DOI: 10.19540/j.cnki.cjcmm.20181009.010

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