高家荣;朱梦情;汪小莉;吴欢;单莉;王云;
GAO Jia-rong;ZHU Meng-qing;WANG Xiao-li;WU Huan;SHAN Li;WANG Yun;State Administration of Chinese Medicine Preparation Three Laboratories, First Affiliated Hospital of Anhui University of Chinese Medicine;Anhui Province Key Laboratory of Chinese Medicinal Formula;School of Pharmacy, Anhui University of Chinese Medicine;the Experimental Research Center, Anhui University of Chinese Medicine;

• 1:安徽中医药大学第一附属医院国家中医药管理局中药制剂三级实验室
• 2:中药复方安徽省重点实验室
• 3:安徽中医药大学药学院
• 4:安徽中医药大学科研实验中心

摘要(Abstract)：

为了明确黄地安消胶囊的主要化学成分,该研究采用超高效液相色谱串联四极杆飞行时间质谱联用技术(UPLC-Q-TOF-MS^E)结合WatersUNIFI软件对黄地安消胶囊的化学成分进行快速定性分析。通过UPLC-Q-TOF-MSE)结合WatersUNIFI软件对黄地安消胶囊的化学成分进行快速定性分析。通过UPLC-Q-TOF-MS^E技术采集黄地安消胶囊的质谱数据,根据UNIFI软件匹配的结果,结合对照品的保留时间、特征碎片和文献数据进行比对,共鉴定和推测出100个化合物,25个归属于葛根、22个归属于黄连、6个归属于麦冬、14个归属于枇杷叶、22个归属于生地黄、15个归属于三七,其中有3个化合物为共有成分。所测得的成分包含黄酮类成分、生物碱类成分、皂苷类成分、有机酸类成分等。该研究系统地分析了黄地安消胶囊的化学成分,为阐明黄地安消胶囊的药效物质基础提供了依据。
In order to clarify the main chemical constituents of Huangdi Anxiao Capsules, an ultra-high performance liquid coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MSE技术采集黄地安消胶囊的质谱数据,根据UNIFI软件匹配的结果,结合对照品的保留时间、特征碎片和文献数据进行比对,共鉴定和推测出100个化合物,25个归属于葛根、22个归属于黄连、6个归属于麦冬、14个归属于枇杷叶、22个归属于生地黄、15个归属于三七,其中有3个化合物为共有成分。所测得的成分包含黄酮类成分、生物碱类成分、皂苷类成分、有机酸类成分等。该研究系统地分析了黄地安消胶囊的化学成分,为阐明黄地安消胶囊的药效物质基础提供了依据。
In order to clarify the main chemical constituents of Huangdi Anxiao Capsules, an ultra-high performance liquid coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS^E) combined with Waters UNIFI software were successfully used to rapidly identify the chemical constituents in Huangdi Anxiao Capsules. The mass spectrometry data of chemical constituents from Huangdi Anxiao Capsules were collected by UPLC-Q-TOF-MSE) combined with Waters UNIFI software were successfully used to rapidly identify the chemical constituents in Huangdi Anxiao Capsules. The mass spectrometry data of chemical constituents from Huangdi Anxiao Capsules were collected by UPLC-Q-TOF-MS^E, and their structures were identified by the results of UNIFI software according to relative retention time of reference standards, MS feature fragments and literature data of each compound. A total of 100 compounds in Huangdi Anxiao Capsules were identified, including 25 compounds from Pueraria Lobate Radix, 22 compounds from Coptis Rhizoma, 6 compounds from Ophiopogonis Radix, 14 compounds from Eriobotryae Folium, 22 compounds from Rehmanniae Radix, and 15 compounds from Notoginseng Radix et Rhizoma. Among them, 3 compounds were common components. These 100 compounds included flavonoids, alkaloids, saponins and organic acids. This study systematically analyzed the chemical composition of Huangdi Anxiao Capsules, so as to provide evidences for defining the chemical material basis of Huangdi Anxiao Capsules.

关键词(KeyWords)： 黄地安消胶囊;UPLC-Q-TOF-MS~E;UNIFI;化学成分
Huangdi Anxiao Capsules;UPLC-Q-TOF-MS~E;UNIFI;chemical constituent

Abstract:

Keywords:

基金项目(Foundation): 国家中医临床研究基地业务建设第二批科研专项(JDZX2015126)

作者(Authors): 高家荣;朱梦情;汪小莉;吴欢;单莉;王云;
GAO Jia-rong;ZHU Meng-qing;WANG Xiao-li;WU Huan;SHAN Li;WANG Yun;State Administration of Chinese Medicine Preparation Three Laboratories, First Affiliated Hospital of Anhui University of Chinese Medicine;Anhui Province Key Laboratory of Chinese Medicinal Formula;School of Pharmacy, Anhui University of Chinese Medicine;the Experimental Research Center, Anhui University of Chinese Medicine;

DOI: 10.19540/j.cnki.cjcmm.20191217.301

参考文献(References)：

• [1] 方朝晖,赵进东,唐庆年,等.黄地安消胶囊对肺热津伤证糖尿病患者血清hs-CRP和ADP的影响[J].中医药临床杂志,2012,24(12):1189.
• [2] 高家荣,郭明飞,方朝晖,等.黄地安消胶囊对高糖高脂诱导的斑马鱼血管病变保护作用研究[J].中国中药杂志,2018,43(21):4317.
• [3] 韩利平,高家荣,李静雯,等.HPLC法同时测定黄地安消胶囊中4种化学成分的含量[J].中医药临床杂志,2018,30(3):475.
• [4] 李钰馨,汪永忠,吴小明,等.超高效液相色谱双波长法同时测定黄地安消胶囊中5种成分的含量[J].中国药学杂志,2015,50(4):362.
• [5] ZHU B J,CAO H T,SUN L M,et al.Metabolomics-based mechanisms exploration of Huang-Lian Jie-Du Decoction on cerebral ischemia via UPLC-Q-TOF/MS analysis on rat serum[J].J Ethnopharmacol,2018,216(8):147.
• [6] MIAO W J,QING W,TAO B,et al.Rapid characterization of chemical constituents and rats metabolites of the traditional Chinese patent medicine Gegen-Qinlian-Wan by UHPLC/DAD/qTOF-MS[J].J Pharm Biomed Anal,2013,72(2):99.
• [7] 殷会敏,黄文哲,肖伟.基于RRLC-Q-TOF-MS/MS技术快速分析芪葛颗粒的化学成分[J].中国中药杂志,2018,43(21):4240.
• [8] ZHANG R X,LI M X,JIA Z P.Rehmannia glutinosa:review of botany,chemistry and pharmacology[J].J Ethnopharmacol,2008,117(2):199.
• [9] WANG H,SUN H,ZHANG A,et al.Rapid identification and comparative analysis of the chemical constituents and metabolites of Phellodendri amurensis cortex and Zhibai dihuang pill by ultra-performance liquid chromatography with quadrupole TOF-MS[J].J Sep Sci,2013,36(24):3874.
• [10] 钟永康.枇杷叶化学成分及提取分离方法的研究进展[J].今日药学,2017,27(9):646.
• [11] 任真真,张燕玲,王星,等.三七皂苷类成分的蛋白质相互作用网络分析[J].中国中药杂志,2014,39(11):2097.
• [12] 王庆慧,李铣,王金辉,等.麦冬化学成分研究[J].中国现代中药,2009,11(11):21.
• [13] ZHANG S,WANG M,WANG C.Preparative separation and purification of alkaloids from Rhizoma Coptidis by high-speed counter-current chromatography[J].Sep Purif Technol,2011,76(3):428.
• [14] 张启云,彭国梅,李冰涛,等.UHPLC-Q-TOF/MS技术分析葛根醇提液中化学成分[J].中药新药与临床药理,2017,28(4):513.
• [15] ZHANG Y Z,XU F,YI T,et al.Investigations of the fragmentation behavior of 11 isoflavones with ESI-IT-TOF-MSn[J].J Chin Pharm Sci,2014,23(9):631.
• [16] 孙冬梅,董玉娟,胥爱丽,等.葛根枳椇软胶囊中黄酮类化合物的UPLC/Q-TOF-MS快速分析[J].中草药,2015,46(7):970.
• [17] 刘梦娇,王云,李玲云,等.三黄片化学成分的UPLC-Q-TOF-MS/MS快速鉴定分析[J].中国中药杂志,2017,42(9):1685.
• [18] TIAN P P,ZHANG X X,WANG H P,et al.Rapid analysis of components in Coptis chinensis Franch by ultra-performance liquid chromatography with quadrupole time-of-flight mass spectrometry[J].Pharmacogn Mag,2017,13(49):175.
• [19] XU X L,LIU Y,WU H F,et al.Rapid identification of chemical profile in Gandou decoction by UPLC-Q-TOF-MSE coupled with novel informatics UNIFI platform[J].J Pharm Anal,2019,doi:10.1016/j.jpha.2019.05.003.
• [20] 李淑娇,王宇卿.HPLC-ABTS-DAD-Q-TOF/MS在线筛选黄连抗氧化活性物质[J].中国中药杂志,2018,43(12):2570.
• [21] CHANG J S L,TING T,SU L Z,et al.An integrated high resolution mass spectrometric data acquisition method for rapid screening of saponins in Panax notoginseng (Sanqi)[J].J Pharm Biomed Anal,2015,109(10):184.
• [22] 赵静,秦振娴,彭冰,等.基于UPLC-Q-TOFMS技术的三七中皂苷类成分质谱裂解规律研究[J].质谱学报,2017,38(1):97.
• [23] 谢晶,张丽,曾金祥,等.基于UPLC-Q-TOF-MS/MS技术的短管兔耳草化学成分快速识别研究[J].中国中药杂志,2017,42(11):2123.
• [24] 张波泳,江振作,王跃飞,等.UPLC/ESI-Q-TOFMS法分析鲜地黄、生地黄、熟地黄的化学成分[J].中成药,2016,38(5):1104.
• [25] LIANG Q D,MA J,MA Z C,et al.Chemical comparison of dried rehmannia root and prepared rehmannia root by UPLC-TOF MS and HPLC-ELSD with multivariate statistical analysis[J].Acta Pharm Sin B,2013,3(1):55.
• [26] 张雯霞,冯敏,苗雨露,等.基于UPLC-Q-TOF-MS/MS技术的还贝止咳方化学成分分析[J].中国中药杂志,2019,44(14):3022.
• [27] 程斯倩,陈雪,于馨洋,等.葛根异黄酮药理作用的研究进展[J].吉林医药学院学报,2013,34(1):46.
• [28] 马航,胡慭然,邹宗尧,等.黄连生物碱降糖作用研究及构效关系初探[J].中国药理学通报,2015,31(11):1575.
• [29] DU Y G,WANG L P,QIAN J W,et al.Panax notoginsengsaponins protect kidney from diabetes by up-regulating silent information regulator 1 and activating antioxidant proteins in rats[J].Chin J Integr Med,2016,22(12):910.
• [30] 李娟,王如锋,杨莉,等.三七皂苷类成分及对心血管作用的研究进展[J].中国中药杂志,2015,40(17):3480.
• [31] 宋光西,马玲云,魏锋,等.苯乙醇苷的分布及药理活性研究进展[J].亚太传统医药,2011,7(4):169.
• [32] 李更生,王慧森,刘明,等.地黄中环烯醚萜苷类化学成分的研究[J].中医研究,2008,21(5):17.
• [33] 谢彩侠,李雅静,张苗,等.不同种质地黄块根菊花心与非菊花心部位有效成分特征分析[J].中国实验方剂学杂志,2018,24(20):75.
• [34] 袁春丽,孙立,袁胜涛,等.麦冬有效成分的药理活性及作用机制研究进展[J].中国新药杂志,2013,22(21):2496.
• [35] 叶长平,商洪涛.关于单味中药枇杷叶研究的思考[J].中医临床研究,2018,10(16):8.
• [36] 胡益勇,徐晓玉.阿魏酸的化学和药理研究进展[J].中成药,2006,28(2):253.
• [37] MA Y,GAO M,LIU D.Chlorogenic acid improves high fat diet-induced hepatic steatosis and insulin resistance in mice[J].Pharm Res,2015,32(4):1200.