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UPLC-Q-TOF-MS~E技术结合UNIFI软件分析黄地安消胶囊化学成分
Identification of chemical constituents in Huangdi Anxiao Capsules by UPLC-Q-TOF-MS~E combined with UNIFI software

高家荣;朱梦情;汪小莉;吴欢;单莉;王云;
GAO Jia-rong;ZHU Meng-qing;WANG Xiao-li;WU Huan;SHAN Li;WANG Yun;State Administration of Chinese Medicine Preparation Three Laboratories, First Affiliated Hospital of Anhui University of Chinese Medicine;Anhui Province Key Laboratory of Chinese Medicinal Formula;School of Pharmacy, Anhui University of Chinese Medicine;the Experimental Research Center, Anhui University of Chinese Medicine;

摘要(Abstract):

为了明确黄地安消胶囊的主要化学成分,该研究采用超高效液相色谱串联四极杆飞行时间质谱联用技术(UPLC-Q-TOF-MSE)结合WatersUNIFI软件对黄地安消胶囊的化学成分进行快速定性分析。通过UPLC-Q-TOF-MSE)结合WatersUNIFI软件对黄地安消胶囊的化学成分进行快速定性分析。通过UPLC-Q-TOF-MSE技术采集黄地安消胶囊的质谱数据,根据UNIFI软件匹配的结果,结合对照品的保留时间、特征碎片和文献数据进行比对,共鉴定和推测出100个化合物,25个归属于葛根、22个归属于黄连、6个归属于麦冬、14个归属于枇杷叶、22个归属于生地黄、15个归属于三七,其中有3个化合物为共有成分。所测得的成分包含黄酮类成分、生物碱类成分、皂苷类成分、有机酸类成分等。该研究系统地分析了黄地安消胶囊的化学成分,为阐明黄地安消胶囊的药效物质基础提供了依据。
In order to clarify the main chemical constituents of Huangdi Anxiao Capsules, an ultra-high performance liquid coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MSE技术采集黄地安消胶囊的质谱数据,根据UNIFI软件匹配的结果,结合对照品的保留时间、特征碎片和文献数据进行比对,共鉴定和推测出100个化合物,25个归属于葛根、22个归属于黄连、6个归属于麦冬、14个归属于枇杷叶、22个归属于生地黄、15个归属于三七,其中有3个化合物为共有成分。所测得的成分包含黄酮类成分、生物碱类成分、皂苷类成分、有机酸类成分等。该研究系统地分析了黄地安消胶囊的化学成分,为阐明黄地安消胶囊的药效物质基础提供了依据。
In order to clarify the main chemical constituents of Huangdi Anxiao Capsules, an ultra-high performance liquid coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MSE) combined with Waters UNIFI software were successfully used to rapidly identify the chemical constituents in Huangdi Anxiao Capsules. The mass spectrometry data of chemical constituents from Huangdi Anxiao Capsules were collected by UPLC-Q-TOF-MSE) combined with Waters UNIFI software were successfully used to rapidly identify the chemical constituents in Huangdi Anxiao Capsules. The mass spectrometry data of chemical constituents from Huangdi Anxiao Capsules were collected by UPLC-Q-TOF-MSE, and their structures were identified by the results of UNIFI software according to relative retention time of reference standards, MS feature fragments and literature data of each compound. A total of 100 compounds in Huangdi Anxiao Capsules were identified, including 25 compounds from Pueraria Lobate Radix, 22 compounds from Coptis Rhizoma, 6 compounds from Ophiopogonis Radix, 14 compounds from Eriobotryae Folium, 22 compounds from Rehmanniae Radix, and 15 compounds from Notoginseng Radix et Rhizoma. Among them, 3 compounds were common components. These 100 compounds included flavonoids, alkaloids, saponins and organic acids. This study systematically analyzed the chemical composition of Huangdi Anxiao Capsules, so as to provide evidences for defining the chemical material basis of Huangdi Anxiao Capsules.

关键词(KeyWords): 黄地安消胶囊;UPLC-Q-TOF-MS~E;UNIFI;化学成分
Huangdi Anxiao Capsules;UPLC-Q-TOF-MS~E;UNIFI;chemical constituent

Abstract:

Keywords:

基金项目(Foundation): 国家中医临床研究基地业务建设第二批科研专项(JDZX2015126)

作者(Authors): 高家荣;朱梦情;汪小莉;吴欢;单莉;王云;
GAO Jia-rong;ZHU Meng-qing;WANG Xiao-li;WU Huan;SHAN Li;WANG Yun;State Administration of Chinese Medicine Preparation Three Laboratories, First Affiliated Hospital of Anhui University of Chinese Medicine;Anhui Province Key Laboratory of Chinese Medicinal Formula;School of Pharmacy, Anhui University of Chinese Medicine;the Experimental Research Center, Anhui University of Chinese Medicine;

DOI: 10.19540/j.cnki.cjcmm.20191217.301

参考文献(References):

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