中国中药杂志

2014, v.39(11) 2121-2125

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荭草提取物的肠外翻吸收研究
Absorption of extractive Polygonum orientale in rat everted gut sacs

刘跃;唐丽;曹旭;郑林;王爱民;黄勇;
LIU Yue;TANG Li;CAO Xu;ZHENG Lin;WANG Ai-min;HUANG Yong;Guizhou Provincial Key Laboratory of Pharmaceutics,School of Pharmacy,Guiyang Medical College;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine,Ministry of Education,Guiyang Medical College;

摘要(Abstract):

利用肠外翻模型研究荭草提取物不同剂量在大鼠不同肠段的体外肠吸收特征。采用UPLC-MS/MS检测不同肠段中原儿茶酸,异荭草素,荭草素,牡荆素,木犀草苷,槲皮苷及山柰素鼠李糖苷含量,比较荭草提取物中各化学成分在各肠段的吸收情况,并计算提取物中各成分的吸收参数,用SPSS 13.0统计学软件对数据进行分析处理,分析考察对吸收参数影响的显著性,进一步分析其在不同部位的吸收特征。荭草提取物不同浓度时原儿茶酸等7种成分在不同肠段的吸收均为线性吸收,其回归相关系数(R2)均大于0.95,符合零级吸收速率,肠吸收速率(Ka)随着荭草提取物浓度增加呈线性增加(R2>0.95),表明各成分以被动扩散机制吸收;所考察成分在不同肠段肠囊的吸收速率存在不同程度的差异,各成分在各肠道的吸收趋势为十二指肠、空肠、回肠大于结肠。肠囊对荭草中化学成分的吸收透过有选择性,外翻肠囊法可评价其多成分的肠吸收特性。
Using in vitro everted gut to investigate the intestinal absorption of the extracts from Polygonum orientale at different concentration.UPLC-MS/MS was used to detect the content of protocatechuic acid,isoorientin,orientin,vitexin,cynaroside,quercitrin,kaempferol-rhamnoside in different intestinal segments,then compared the results with the absorption of chemical components of extractive P.orientale in each intestinal segments,and calculated the absorption parameter.We took the statistic analysis with SPSS statistic software.The influence significance of each factors were analyzed to describe the character of absorption.The absorption of each component is linearity in different intestinal segments and different dose,and the square of coeficient correlation exceed 0.95,which consistent with zero order rate process.The Kaincrease along with the raised dosage of the extractive P.orientale(R2> 0.95),indicated it is the passive absorption; different intestinal segments have different absorption.And the absorption trend in intestinal is duodenum,jejunum,ileum are greater than the colon.As ingredients are selectively absorbed in intestinal sac,the everted intestinal sac method is selected to assess the intestinal absorption charcteristics of ingredients of extractive P.orientale.

关键词(KeyWords): 荭草;肠外翻;吸收;UPLC-MS/MS;吸收速率常数
Polygonum orientale;in vitro everted intestinal sac;absorption;UPLC-MS/MS;absorption rate constant

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(81360664);; 贵州省中药现代化科技产业研究开发专项项目(黔科合中药字[2011]5081,黔科合重G字[2013]4001)

作者(Author): 刘跃;唐丽;曹旭;郑林;王爱民;黄勇;
LIU Yue;TANG Li;CAO Xu;ZHENG Lin;WANG Ai-min;HUANG Yong;Guizhou Provincial Key Laboratory of Pharmaceutics,School of Pharmacy,Guiyang Medical College;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine,Ministry of Education,Guiyang Medical College;

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