史雅红;任建勋;刘建勋;黄婷婷;郝婷婷;王敏;李军梅;林成仁;任钧国;
SHI Ya-hong;REN Jian-xun;LIU Jian-xun;HUANG Ting-ting;HAO Ting-ting;WANG Min;LI Jun-mei;LIN Cheng-ren;REN Jun-guo;Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences;

• 1:中国中医科学院西苑医院基础医学研究所中药药理北京市重点实验室

摘要(Abstract)：

运用睡眠剥夺法诱导大鼠血瘀证模型,检测中医证候宏观指标体质量、舌象及血液流变学确定中医血瘀证候,运用酶联免疫法检测血小板α颗粒、致密颗粒释放及活化相关指标,为证候研究的血小板功能评价及相关药物影响提供实验基础。结果显示,模型组大鼠体质量较正常组明显降低(P<0.01);舌象呈现紫暗的血瘀证特点,色彩分析舌面R,G,B较正常组明显降低(P<0.01);血液流变学高切、中切、低切全血黏度均高于正常组(P<0.01),血浆黏度未有明显变化;血小板α颗粒(GMP-140,β-TG,PF4)及致密颗粒(ADP,5-HT)释放明显(P<0.01)。血浆TXB_2和6-keto-PGF1α都较正常组明显升高(P<0.01),且两者比值也显著高于正常组(P<0.01)。PAF模型组较正常组明显上升(P<0.01)。结果表明睡眠剥夺诱导的大鼠血瘀证血小板功能存在明显改变,血小板活化增强,颗粒释放增多,并可能存在炎症及内皮细胞功能异常。
Blood stasis syndrome is the pre-state of thrombotic disease. The model of blood stasis syndrome in rats was induced by sleep deprivation to study on effects of blood stasis syndrome on platelet activation. The weight,the color of tongue and hemorheology for the blood stasis syndrome of Chinese medicine were measured after modeling. The release of platelet granules and platelet activation factors in plasma were detected by ELISA kit related indicators to provide experimental basis for platelet function evaluation and related drug effects in syndrome research. The results showed that the weight of the model group rats was significantly lower than that of the normal group( P < 0. 01). The tongue showed a dark purple blood stasis pattern,and the R,G and B values of the tongue surface in model group were significantly lower than those of the normal group( P < 0. 01). The hemorheological parameters including high shear,middle shear and low shear viscosity in whole blood were significantly higher than those in the normal group( P < 0. 01). But plasma viscosity did not change significantly. The release levels of platelet α particles( GMP-140,β-TG,PF4) and dense particles( ADP,5-HT) were significantly higher than those in the normal group( P < 0. 01). The levels of TXB_2 and 6-keto-PGF1 αin plasma were significantly higher than those in the normal group( P < 0. 01). The ratios of TXB_2 and 6-keto-PGF1 αwere also significantly higher than those in the normal group( P < 0. 01). The levels of PAF in plasma in model group were significantly higher than those in the normal group( P < 0. 01). It was concluded that platelet functions could be changed induced by sleep deprivationin rats with blood stasis syndrome,and there might be inflammation and endothelial cell dysfunction.

关键词(KeyWords)： 睡眠剥夺;血瘀;证候;血小板;颗粒释放
sleep deprivation;blood stasis;syndrome;platelet;granule release

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划(973)项目(2015CB554405)

作者(Authors): 史雅红;任建勋;刘建勋;黄婷婷;郝婷婷;王敏;李军梅;林成仁;任钧国;
SHI Ya-hong;REN Jian-xun;LIU Jian-xun;HUANG Ting-ting;HAO Ting-ting;WANG Min;LI Jun-mei;LIN Cheng-ren;REN Jun-guo;Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences;

DOI: 10.19540/j.cnki.cjcmm.20180522.006

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