中国中药杂志

2009, v.34(24) 3235-3238

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UPLC测定鸡眼睛药材中鞣花酸类化合物的含量
Determination of ellagic acid compounds in Euscaphis japonica by using UPLC

王爱民;刘智;王海军;李勇军;廖尚高;王永林;
WANG Aimin1,LIU Zhi2,WANG Haijun1,LI Yongjun1,LIAO Shanggao1,WANG Yonglin1* (1.School of Pharmacy,Guiyang Medical Colledge,Guiyang 550004,China;2.Guizhou EAKAN Pharmaceutical Co.,Ltd,Guiyang 550008,China)

摘要(Abstract):

目的:建立超高效液相色谱(UPLC)法测定鸡眼睛(野鸦椿)中鞣花酸类化合物含量的方法。方法:采用AcquityBEHC18(2.1 mm×100 mm,1.7μm)色谱柱;以乙腈-0.1%磷酸为流动相,梯度洗脱;流速0.2 mL.min-1;检测波长245 nm。结果:鸡眼睛药材中5种鞣花酸类化合物在20 min内得到完全分离并进行了含量测定。方法快速、专属,线性关系、精密度和准确度良好。结论:通过研究制定了鸡眼睛(野鸦椿)药材中鞣花酸类化合物的质量控制方法。
Objective: To develop an ultra performance liquid chromatography(UPLC) method for simultaneous determination of five ellagic acids in Euscaphis japonica.Method: Analysis was carried out on an Acquity BEH C18(2.1 mm×100 mm,1.7 μm) column at 40 ℃ eluted with acetonitrile-0.1% phosphoric acid aqueous solution as mobile phases in gradient elution.The flow rate was 0.2 mL·min-1,and the detection wavelength set was monitored at 245 nm.Result: All calibration curves showed good linear relationship within test ranges(r>0.999 7),and the overall recoveries were in the range of 97.2%-102.1%,with RSD less than 3.2%(n=6).The overall RSD of precision test were less than 2.9%.Conclusion:The developed method was simple,rapid,accurate and reproducible,and can be used for the quality control of E.japonica.

关键词(KeyWords): 鸡眼睛(野鸦椿);鞣花酸类;超高液相色谱(UPLC)
Euscaphis japonica;ellagic acid compounds;UPLC

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基金项目(Foundation): 贵州省中药现代化科技产业研究开发专项基金项目(黔科合中药专字[2007]5014号)

作者(Author): 王爱民;刘智;王海军;李勇军;廖尚高;王永林;
WANG Aimin1,LIU Zhi2,WANG Haijun1,LI Yongjun1,LIAO Shanggao1,WANG Yonglin1* (1.School of Pharmacy,Guiyang Medical Colledge,Guiyang 550004,China;2.Guizhou EAKAN Pharmaceutical Co.,Ltd,Guiyang 550008,China)

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