中国中药杂志

2016, v.41(01) 118-123

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芪蛭降糖胶囊对2型糖尿病大鼠皮肤创面愈合的影响
Effects of Qizhi Jiangtang capsule on dermal ulcer in type 2 diabetic rats

刘博;于春江;孟祥宝;王鑫;赵宏伟;孙桂波;纪凤兰;徐惠波;孙晓波;
LIU Bo;YU Chun-jiang;MENG Xiang-bao;WANG Xin;ZHAO Hong-wei;SUN Gui-bo;JI Feng-lan;XU Hui-bo;SUN Xiao-bo;Heilongjiang University of Chinese Medicine;Academy of Chinese Medical Sciences of Jilin Province;Jilin YiZheng Pharmaceutical Group Co.,Ltd.;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences;

摘要(Abstract):

雄性Wistar大鼠,除预留空白组大鼠外,其余动物通过饲喂高糖高脂饲料4周并腹腔注射30 mg·kg-1链脲佐菌素(STZ)造成2型糖尿病模型后,按血糖均衡分组并给予相应药物治疗8周,8周末于大鼠背部做直径2 cm圆形切口。继续给药10 d,期间每2 d测量创面面积,10 d后剪下创面肉芽组织用于病理学检查及血管内皮生长因子(VEGF)、磷脂酰肌醇-3激酶(PI3K)、磷酸化的胞外调节蛋白激酶(p-ERK)蛋白在创面组织的表达情况。结果显示,与模型组相比,芪蛭降糖胶囊(2.24g·kg-1)皮肤创面面积在创面形成第6天和第10天明显减小;溃疡面炎症反应明显减轻;芪蛭降糖胶囊能提高糖尿病大鼠皮肤创面组织中VEGF蛋白表达,抑制ERK的磷酸化,提示芪蛭降糖胶囊可以促进糖尿病皮肤溃疡的愈合,其机制可能与调节VEGF和p-ERK蛋白的表达有关。
The effect of Qizhi Jiangtang vapsule( QJC) on degree of dermal ulcer cicatrization in 2 type diabetic rats was studied.Except the rats for blank group,other male Wistar rats were used to establish type 2 diabetic model by feeding with high sugar and high fat diet for four weeks and intraperitonally injecting with 30 mg·kg~(-1) streptozotocin( STZ). After that,the rats were divided into balanced groups according to blood sugar,and received corresponding drugs for treatment for 8 weeks. At the end of week 8,2 cm diameter circular incision was done on the back of rats. After that,the rats were administered continuously for10 days. Area of ulcer surface was detected every two days. After the last administration,wound granulation tissues were cut down to conduct pathological examination and detect the expression of VEGF,PI3 K,p-ERK protein in wound tissues. The results showed that compared with the model group,after application of Qizhi Jiangtang capsule( 2. 24 g·kg~(-1)),the wound was significantly reduced on day 6 and day 10 of wound formation; inflammation reaction on ulcer surface was significantly reduce; Qizhi Jiangtang capsule can increase VEGF expression in the wound tissues of diabetic rats,and inhibit ERK phosphorylation. It can be concluded that Qizhi Jiangtang capsule can promote skin ulcer healing for diabetes rats,and its mechanism may be related to regulating the expression of VEGA and p-ERK proteins.

关键词(KeyWords): 芪蛭降糖胶囊;2型糖尿病;糖尿病溃疡
Qizhi Jiamgtang capsule;type 2 diabetes mellitus;diabetic ulcer

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2011ZX09201-201-31,2012ZX09501001-004)

作者(Author): 刘博;于春江;孟祥宝;王鑫;赵宏伟;孙桂波;纪凤兰;徐惠波;孙晓波;
LIU Bo;YU Chun-jiang;MENG Xiang-bao;WANG Xin;ZHAO Hong-wei;SUN Gui-bo;JI Feng-lan;XU Hui-bo;SUN Xiao-bo;Heilongjiang University of Chinese Medicine;Academy of Chinese Medical Sciences of Jilin Province;Jilin YiZheng Pharmaceutical Group Co.,Ltd.;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences;

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