中国中药杂志

2010, v.35(19) 2576-2580

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RP-HPLC测定不同产地黄连中6种生物碱的含量
Simultaneous determination of six alkaloids in Coptis chinensis of different regions by RP-HPLC

耿志鹏;郑海杰;张艺;罗维早;瞿显友;
GENG Zhipeng1, ZHENG Haijie1, ZHANG Yi1, LUO Weizao2, QU Xianyou2(1. College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;2.Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China)

摘要(Abstract):

目的:建立RP-HPLC同时测定黄连中药根碱、非洲防己碱、表小檗碱、黄连碱、巴马汀及小檗碱等6种生物碱含量的方法。方法:XtimateTM C18色谱柱(4.6mm×250mm,5μm);流动相为乙腈-30mmol·L-1碳酸氢铵水溶液(含有0.7%氨水、0.1%三乙胺),线性梯度洗脱;检测波长270nm;柱温30℃;流速1.0mL·min-1。结果:6种生物碱分别在0.85~16.96(r=0.9997),1.25~24.96(r=0.9995),2.05~40.96(r=0.9999),3.65~72.96(r=0.9999),2.88~57.60(r=0.9998),13.25~264.96mg·L-1(r=0.9996)具有良好的线性;平均加样回收率(n=6)依次为101.6%(RSD1.3%),102.5%(RSD1.5%),100.8%(RSD1.9%),102.6%(RSD1.2%),97.80%(RSD1.3%),99.01%(RSD1.5%)。不同产地黄连中6种被测生物碱及总生物碱存在显著的差异。结论:该方法准确、可靠,重复性好,可用于同时测定黄连中药根碱、非洲防己碱、表小檗碱、黄连碱、巴马汀及小檗碱的含量。
A reversed-phase HPLC method for simultaneous determination of gatrorrhizine, columbamine, epiberberine, coptisine, palmatine and berberine in Coptis chinensis was developed. Analysis was carried out on an XtimateTM C18 column (4.6 mm×250 mm, 5 μm)eluted with acetonitrile-30 mmol·L-1 ammonium bicarbonate solution(including 0.7% ammonia and 0.1% triethylamine) by gradient elution. The detective wavelength was 270 nm, the column temperature was 30 ℃, and the flow rate was 1.0 mL·min-1. By the above method, the linear ranges of gatrorrhizine, columbamine, epiberberine, coptisine, palmatine and berberine were 0.85-16.96 (r=0.999 7), 1.25-24.96 (r=0.999 5), 2.05-40.96 (r=0.999 9),3.65-72.96 (r=0.999 9),2.88-57.60 (r=0.999 8),13.25-264.96 mg·L-1(r=0.999 6), respectively. The average recoveries (n=6) of the six alkaloids were 101.6%(RSD 1.3%),102.5%(RSD 1.5%),100.8%(RSD 1.9%),102.6%(RSD 1.2%),97.80%(RSD 1.3%),99.01%(RSD 1.5%), respectively. The determined results demonstrate that there is a significant difference in the contents of six alkaloids and total alkaloids among the tested samples. The method is accurate, reliable and repeatable for simultaneous determination of gatrorrhizine, columbamine, epiberberine, coptisine, palmatine and berberine in C. chinensis.

关键词(KeyWords): 黄连;药根碱;非洲防己碱;表小檗碱;黄连碱;巴马汀;小檗碱;RP-HPLC
Coptis chinensis; gatrorrhizine; columbamine; epiberberine; coptisine; palmatine; berberine; RP-HPLC

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基金项目(Foundation): 国家“十一五”科技支撑计划项目(2007BAI40B05;2007BAI40B03)

作者(Author): 耿志鹏;郑海杰;张艺;罗维早;瞿显友;
GENG Zhipeng1, ZHENG Haijie1, ZHANG Yi1, LUO Weizao2, QU Xianyou2(1. College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;2.Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China)

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