中国中药杂志

2013, v.38(05) 687-690

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HPLC法同时测定小儿平喘祛痰颗粒中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷含量
Simultaneous determination of ephedrine hydrochloride,D-pseudoephedrine and amygdalin in Xiao′er Pingchuan Qutan granule by HPLC

杨德斌;仝燕;马振山;王琳;董美虹;李艳玲;王锦玉;
YANG De-bin1,2,TONG Yan1,MA Zhen-shan1,WANG Lin1, DONG Mei-hong1,2,LI Yan-ling1,2,WANG Jin-yu1*(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 2.Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China)

摘要(Abstract):

目的:建立同时测定小儿平喘祛痰颗粒中3种成分含量的高效液相色谱法。方法:采用Phenyl Ether色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%磷酸溶液(含0.1%的三乙胺)(3∶97),紫外检测波长210 nm,流速1.0 mL.min-1,柱温35℃。结果:盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷的线性范围分别为0.078 60~3.144μg(r=1.000 0),0.103 4~2.068μg(r=0.999 7),0.430 5~3.157μg(r=0.999 8);平均回收率分别为98.46%(RSD 1.1%),103.0%(RSD1.5%),97.15%(RSD 2.1%)。结论:该法简便、稳定、可靠,可以用于小儿平喘祛痰颗粒中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷的含量测定。
Objective: To establish an HPLC method for the determination of ephedrine hydrochloride,D-pseudo-ephedrine and amygdalin in Xiao′er Pingchuan Qutan granule.Method: Pheny ether chromatographic column(4.6 mm×250 mm,5 μm) was adopted,with acetonitrile-0.1% phosphoric acid(containing 0.1% three ethylamine)(3∶97) as the mobile phase.The UV detection wavelength was at 210 nm,with the flow rate of 1 mL·min-1,and column temperature was at 35 ℃.Result: The linearity of ephedrine hydrochloride,D-pseudo-ephedrine and amygdalin ranged between 0.078 60-3.144 μg(r=1.000 0),0.103 4-2.068 μg(r=0.999 7) and 0.430 5-3.157 μg(r=0.999 8),respectively.Their average recoveries were 98.46%(RSD 1.1%),103.0%(RSD 1.5%) and 97.15%(RSD 2.1%),respectively.Conclusion: The method is simple,stable and reliable that it can be used to determine the content of ephedrine hydrochloride,D-pseudo-ephedrine and amygdalin in Xiao′er Pingchuan Qutan granule.

关键词(KeyWords): 高效液相色谱;小儿平喘祛痰颗粒;盐酸麻黄碱;盐酸伪麻黄碱;苦杏仁苷
HPLC;Xiao′er Pingchuan Qutan granule;ephedrine hydrochloride;D-pseudo-ephedrine;amygdalin

Abstract:

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基金项目(Foundation): 中国中医科学院基本科研业务费自主选题项目(2011XYCZ-07)

作者(Author): 杨德斌;仝燕;马振山;王琳;董美虹;李艳玲;王锦玉;
YANG De-bin1,2,TONG Yan1,MA Zhen-shan1,WANG Lin1, DONG Mei-hong1,2,LI Yan-ling1,2,WANG Jin-yu1*(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 2.Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China)

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