中国中药杂志

2016, v.41(15) 2824-2829

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斑蝥水提液中斑蝥素与斑蝥酸的稳定性研究
Stability of cantharidin and cantharidic acid in Mylabris aqueous solution

蒋颖;刘红波;曹琴;朱振华;史乙伟;钱大玮;唐志书;赵步长;段金廒;
JIANG Ying;LIU Hong-bo;CAO Qin;ZHU Zhen-hua;SHI Yi-wei;QIAN Da-wei;TANG Zhi-shu;ZHAO Bu-chang;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine;Shaanxi University of Chinese Medicine;Shandong Buchang Pharmaceutical Company Limited;

摘要(Abstract):

研究斑蝥水提液中斑蝥素与斑蝥酸在不同条件下的稳定性及转化规律。采用UPLC-TQ-MS分别测定不同pH、不同温度及光照条件下斑蝥水提液中斑蝥素与斑蝥酸的含量。结果显示,斑蝥水提液中斑蝥素的含量随pH的上升而逐渐降低,而其中斑蝥酸的含量变化则相反;斑蝥水提液于不同pH条件下,分别置于25,40℃及25℃光照90 d,定时取样分析,结果显示其中斑蝥素与斑蝥酸在起始的10 d内含量变化较大,具体表现为酸性溶液中斑蝥酸含量的下降与斑蝥素含量的上升,碱性溶液中斑蝥酸含量的上升与斑蝥素含量下降。此后,各条件下两者的含量基本不变。研究表明pH是影响斑蝥水提液中斑蝥素与斑蝥酸含量的主要因素,两者随溶液p H的改变而相互转化,高温与光照可加快这种转化的平衡速度。该研究结果可为以斑蝥药材为原料的制剂的制备工艺质量控制提供一定的参考。
To investigate the stability and the conversion rules of cantharidin and cantharidic acid contained in the Mylabris aqueous solution under different conditions. The contents of cantharidin and cantharidic acid under different conditions( p H,temperature and light) were determined by HPLC-TQ-MS. The results showed that the content of cantharidin was gradually decreased with the rising of p H value,while on the contrary,the content of cantharidic acid was increased gradually; after Mylabris aqueous solution with different p H values were placed at 25,40 ℃ and 25 ℃ respectively for lighting for 90 days,the samples were collected for analysis. The results showed the contents of cantharidin and cantharidic acid were changed greatly in the first 10 days,mainly including the decrease of cantharidic acid and increase of cantharidin when the solution was acidic,and the increase of cantharidic acid and decrease of cantharidin when the solution was alkaline. After that,the contents of the above two components basically remained stable. This study revealed that p H was the main factor to affect the contents of cantharidin and cantharidic acid,and they could be converted into each other with the changes of p H value. High temperature and light can accelerate the speed of achieving such balance. The study can provide certain reference for the quality control of the medicines using the Mylabris as raw material.

关键词(KeyWords): 斑蝥素;斑蝥酸;UPLC-TQ-MS;稳定性
cantharidin;cantharidic acid;UPLC-TQ-MS;stability

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基金项目(Foundation): 江苏高校优势学科建设工程项目(ysxk-2014)

作者(Author): 蒋颖;刘红波;曹琴;朱振华;史乙伟;钱大玮;唐志书;赵步长;段金廒;
JIANG Ying;LIU Hong-bo;CAO Qin;ZHU Zhen-hua;SHI Yi-wei;QIAN Da-wei;TANG Zhi-shu;ZHAO Bu-chang;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine;Shaanxi University of Chinese Medicine;Shandong Buchang Pharmaceutical Company Limited;

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