中国中药杂志

2019, v.44(08) 1545-1551

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基于高通量测序的紫花丹参与白花丹参根际细菌群落结构研究
Community structure and diversity of soil bacteria in rhizosphere of Salvia miltiorrhiza and Salvia miltiorrhiza f. alba based on pyrosequencing

蒋靖怡;王铁霖;池秀莲;吕朝耕;蒋待泉;王升;康传志;张燕;黄璐琦;郭兰萍;孙楷;
JIANG Jing-yi;WANG Tie-lin;CHI Xiu-lian;LYU Chao-geng;JIANG Dai-quan;WANG Sheng;KANG Chuan-zhi;ZHANG Yan;HUANG Lu-qi;GUO Lan-ping;SUN Kai;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

根际细菌在提高植物营养吸收、促进植株生长及提高植物根系抗病性等方面起着重要作用。该研究以同一地块紫花丹参与白花丹参根际细菌为研究对象,采用16S r DNA高通量测序技术研究其群落结构特征与差异性物种,并分析其优势菌群的功能。结果表明,紫花丹参根际的鞘脂杆菌目、鞘脂单胞菌目、亚硝化单胞菌科等优势菌群丰度显著高于白花丹参。丹参根际优势促生细菌的功能主要集中于促进根际土壤养分的转化、降解自毒物质以及提高植物抗环境胁迫能力。该研究首次比较分析了紫花丹参与白花丹参根际细菌群落结构与差异性物种组成,为进一步研究丹参根际微生态过程提供了科学依据。
Rhizosphere bacteria play a vital role in plant nutrition absorption,growth and disease resistance. In this study,high-throughput sequencing technology was used to analyze the rhizosphere bacterial communities of Salvia miltiorrhiza and S. miltiorrhiza f. alba. Moreover,the function of dominant rhizosphere bacterial communities was analyzed. We found that Sphingobacteriales,Sphingomonadales and Nitrosomonadaceae were both dominant and specific bacteria in the rhizosphere of S. miltiorrhiza. The main functions of dominant rhizosphere bacteria communities in both species include promoting transformation of soil nutrients,improving plant immunity and ability of stress tolerance. This study was the first to compare rhizobacterial communities structure and function of S. miltiorrhiza and S. miltiorrhiza f. alba,which provided a new theoretical reference for studing the rhizosphere mechanism of healthy S. miltiorrhiza planting in the future.

关键词(KeyWords): 紫花丹参;白花丹参;根际;土壤细菌;群落结构功能
Salvia miltiorrhiza;Salvia miltiorrhiza f. alba;rhizosphere;soil bacteria;community structure and function

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2017YFC1700701,2018YFD0201100);; 国家自然科学基金重大项目(81891014);; 中国中医科学院重点领域研究项目(ZZ10-027);中国中医科学院中药资源中心自主选题研究项目(ZZXT201705);; 中央本级重大增减支项目(2060302)

作者(Author): 蒋靖怡;王铁霖;池秀莲;吕朝耕;蒋待泉;王升;康传志;张燕;黄璐琦;郭兰萍;孙楷;
JIANG Jing-yi;WANG Tie-lin;CHI Xiu-lian;LYU Chao-geng;JIANG Dai-quan;WANG Sheng;KANG Chuan-zhi;ZHANG Yan;HUANG Lu-qi;GUO Lan-ping;SUN Kai;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

Email:

DOI: 10.19540/j.cnki.cjcmm.20190301.015

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