中国中药杂志

2008, (18) 2102-2104

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RP-HPLC测定几种花类药材黄酮含量
RP-HPLC determination of flavonoids in several flowers

牛迎凤;邵赟;赵晓辉;文怀秀;陶燕铎;
NIU Ying-feng1,2,SHAO Yun1,ZHAO Xiao-hui1,WEN Huai-xiu1,TAO Yan-duo1 (1.Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China;2.Graduate School of Chinese Academy of Sciences,Beijing 100049,China)

摘要(Abstract):

目的:为充分利用花类药材,建立马蔺花、桃花、月季等15种花类药材中黄酮含量测定的RP-HPLC方法,并比较它们的含量。方法:花类药材用甲醇提取,反相高效液相色谱法分析药材中槲皮素、山柰酚、异鼠李素3种黄酮苷元。结果:采用Kromasil C18柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸(50∶50)为流动相,可使3种黄酮苷元达到基线分离,其中马蔺花含槲皮素1.536%,桃花含山柰酚0.572%,茶花含异鼠李素0.029%,皆为听测样品中最高。结论:本实验首次采用RP-HPLC法测定这15种花类药材的总黄酮含量,本方法操作简便,快速,准确,可作为黄酮的定量分析方法。
Objective: To develvp a RP-HPLC method for the determination of flavonoids in fifteen kinds of flowers such as Iris lacteal pall,prunus persica and rosa chinensis.Method: The contents of quercetin,kaempferol and isorhamntin in fifteen kinds of flowers were extracted with methanol.The analysis was performed on a Kromasil C18 column(4.6 mm×250 mm,5 μm) with methanol-0.1% phosphoric acid(50∶50) as mobile phase.Result: The quercetin,kaempferol and isorhamntin were separated well,and the result shows that the content of quercetin in the Iris lactea Pall was the highest(1.536%),the contene of kaempferol in Persica persice was the highest(0.572%),and the content of isorhamntin in chrysamthemum morifolium was up to 0.290%.Conclusion: The contents of flavonoids in these flowers were by determined RP-HPLC for the first time and the method can be used for quantitative determination of flavonoids in the flowers.

关键词(KeyWords): RP-HPLC;槲皮素;山柰酚;异鼠李素;含量测定
RP-HPLC;quercetin;kaempferol;isorhamntin;determination

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基金项目(Foundation): 青海省重点科技攻关(2007-N-136)

作者(Author): 牛迎凤;邵赟;赵晓辉;文怀秀;陶燕铎;
NIU Ying-feng1,2,SHAO Yun1,ZHAO Xiao-hui1,WEN Huai-xiu1,TAO Yan-duo1 (1.Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China;2.Graduate School of Chinese Academy of Sciences,Beijing 100049,China)

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