中国中药杂志

1998, (06) 3-5

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高效液相色谱法测定家兔血浆中赤芍_(801)的浓度
Determination of Content of Radix Paeoniae Rubar(Chishao) 801 in Rabbit's Plasma by HPLC

王跃生,李计萍,杜贵友,王秀荣,崔海峰,赵雍
Wang Yuesheng, Li Jiping, Du Guiyou, Wang Xiurong, Cui Haifeng and Zhao Yong (Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700)

摘要(Abstract):

用反相高效液相色谱法测定家兔血浆中赤芍801PG浓度。采用NucieoselC18色谱柱,甲醇∶水∶冰乙酸(48∶50∶2)为流动相,4羟基苯甲酸乙酯为内标物,紫外检测波长275nm,对ivPG后不同时间家兔血浆中药物含量进行测定。结果表明:PG浓度为2.5μg/ml的平均回收率为96.70%,其RSD=1.83%。浓度5μg/ml的样品平均回收率为98.86%,RSD=1.05%。其最低检测限为0.1μg/ml。
A HPLC method was used to determine the content of Radix Paeoniae Rubar (Chishao) 801 in rabbit's plasma, using Nucleosil C 18 column, methanol, water and glacial acetic acid (48∶50∶2) as mobile phase ethye 4 hydroxybenzoate as internal standard,and UV detector(λ max ∶275nm, AUFS∶0.02).The average recovery was 96.70% (C:2.5μg/ml) and 98.86%(C: 5μg/ml) respectively. The lowest determination limit was 0.1 μg/ml.The method is simple, accurate, sensitive and reliable.

关键词(KeyWords): 赤芍_(801),没食子酸丙酯,4-羟基苯甲酸乙酯,高效液相色谱法
Chishao 801 ; propyl gallate; 4 hydroxybenzoate; HPLC

Abstract:

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基金项目(Foundation): 国家自然科学基金

作者(Author): 王跃生,李计萍,杜贵友,王秀荣,崔海峰,赵雍
Wang Yuesheng, Li Jiping, Du Guiyou, Wang Xiurong, Cui Haifeng and Zhao Yong (Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700)

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