中国中药杂志

2016, v.41(22) 4188-4193

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拮抗黄芪根腐病菌的根际促生菌的室内筛选与鉴定
In vitro screening and identification of plant growth-promoting rhizobacteria against pathogen causing Astragalus root rot

高芬;郝锐;秦雪梅;雷振宏;王玉龙;王梦亮;
GAO Fen;HAO Rui;QIN Xue-mei;LEI Zhen-hong;WANG Yu-long;WANG Meng-liang;Institute of Applied Chemistry,Shanxi University;Institute of Biotechnology,Shanxi University;Modern Research Center for Traditional Chinese Medicine,Shanxi University;Shanxi Zhendong Geo-herbals Development Co.,Ltd.;

摘要(Abstract):

该文以黄芪根腐病菌Fusarium solani为靶标,采用平板对峙和牛津杯法对被筛菌株进行了抑菌活性评价,并测定了抑菌效果显著且稳定性良好菌株的促生性能,结果显示:菌株G10的活体和发酵液均表现出了明显且稳定的抑菌效果;同时,还具有溶磷能力、固氮能力、淀粉酶活性、产IAA和产HCN等5项促生长特性。形态学观察、生理生化特性测定、16S rDNA序列同源性分析、以及BBL CrystalTM细菌鉴定仪鉴定结果均表明:菌株G10为枯草芽孢杆菌Bacillus subtilis。综上,枯草芽孢杆菌G1 0具备了多功能菌株的基本特征,是开发黄芪根腐病专用微生物农药的有效物质基础。
The antagonistic effect of Bacillus spp. against Fusarium solani was evaluated by living body dual culture and Oxford cup method. The plant growth promoting properties of those strains that had obvious and stable antifungal activity were then tested. The results showed that the living body and bacteria-free fermentation filtrate of strain G10 both had obvious and stable antifungal effect to F. solani. Besides,the strain possessed such growth promoting properties as phosphate solubilization,nitrogen fixation,and production of IAA,amylase and HCN. Strain G10 was classified and identified as B. subtilis by a combination of morphological,physiological and biochemical tests,16 Sr DNA gene sequence analysis and the BBL CrystalTMbacteria identification. In conclusion,B. subtilis G10 has the basic characteristics of multifunctional strains and could be one of the microbiological resources for developing special bio-control agent against Astragalus root rot.

关键词(KeyWords): 黄芪根腐病;植物根际促生菌;筛选;鉴定
Astragalus root rot;plant growth promoting rhizobacteria(PGPR);screening;identification

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基金项目(Foundation): 山西省中药现代化关键技术研究振东专项(2014ZD0501-2)

作者(Author): 高芬;郝锐;秦雪梅;雷振宏;王玉龙;王梦亮;
GAO Fen;HAO Rui;QIN Xue-mei;LEI Zhen-hong;WANG Yu-long;WANG Meng-liang;Institute of Applied Chemistry,Shanxi University;Institute of Biotechnology,Shanxi University;Modern Research Center for Traditional Chinese Medicine,Shanxi University;Shanxi Zhendong Geo-herbals Development Co.,Ltd.;

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