中国中药杂志

2018, v.43(12) 2575-2579

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金银花种质资源化学指纹图谱及代谢物相似性分析
Chemical fingerprinting and similarity analysis of Lonicera japonica resources

朱凤洁;杨健;袁媛;金艳;赵玉洋;黄璐琦;
ZHU Feng-jie;YANG Jian;YUAN Yuan;JIN Yan;ZHAO Yu-yang;HUANG Lu-qi;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

应用LC-MS联用技术对不同产地的41个金银花种质进行检测,建立了金银花种质资源化学指纹图谱并确定23个共有峰,根据对照品和参考文献信息指认了17个共有峰。采用SPSS(version 22.0)软件对获得的41个金银花种质UPLC-MS指纹图谱共有峰峰面积进行系统聚类分析,其变异范围在0.99~0.12。在此基础上,对金银花不同种质代谢物谱一致性评级,并结合遗传一致性分级进行综合评价,为金银花优良种质选育、药材道地性形成机制和评价研究提供基础。
LC-MS was used to detect 41 population of Lonicera japonica from different areas. LC-MS chemical chromatographic profile has been established. There were 23 common peaks,seventeen of which were identified according to reference standard and reference; SPSS software was applied to calculate the similarity of chemical fingerprints of 41 batches and the range was from 0. 99 to 0. 12.On this basis,the L. japonica's metabolites consistency was classified. Combined with comprehensive analysis of genetic identity,we can provide a theoretical basis for the authenticity research of Dao-di herbs and reference information for the breeding of excellent L. japonica.

关键词(KeyWords): 金银花;化学指纹图谱;代谢物谱一致性;双分子标记法
Lonicerae Japonicae Flos;chemical fingerprint;metabolic profile consistency;BIMM

Abstract:

Keywords:

基金项目(Foundation): 科技基础性工作专项(2015FY111500);; 中央级公益性科研院所基本科研业务费专项(ZZ10-008)

作者(Author): 朱凤洁;杨健;袁媛;金艳;赵玉洋;黄璐琦;
ZHU Feng-jie;YANG Jian;YUAN Yuan;JIN Yan;ZHAO Yu-yang;HUANG Lu-qi;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

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DOI: 10.19540/j.cnki.cjcmm.2018.0081

参考文献(References):

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