中国中药杂志

2010, v.35(23) 3103-3107

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明党参居群间根中水溶性成分HPLC分析
Analysis on water-soluble components in roots of Changium smyrnioides among different populations by HPLC

王长林;郭巧生;程搏幸;杨丽雯;周廷惠;
WANG Changlin,GUO Qiaosheng,CHENG Boxing,YANG Liwen,ZHOU Tinghui(Institute of Chinese Medicinal Materials,Nanjing Agricultural University,Nanjing 210095,China)

摘要(Abstract):

目的:分析我国明党参主要分布区内明党参居群间根中水溶性成分的相似性,为明党参种质资源评价及药材质量控制提供理论依据。方法:运用冷浸法和HPLC对10个居群明党参根中水溶性成分进行提取与分析,根据各居群明党参根中主要水溶性成分的相对含量,由夹角余弦法计算相似系数,采用UPGMA方法进行聚类,构建树状图。结果:明党参居群间根中水溶性成分差异显著。九华山居群水溶性浸出物的含量最高,大龙山和浮山2个居群水溶性浸出物的含量低于《中国药典》标准。各居群根中水溶性成分HPLC图谱差异明显,共有峰数目少。居群间根中水溶性成分的相似系数差距很大,为0.030 6~0.999 5。紫金山居群根中水溶性成分最为特殊,除与红山居群的相似系数达到0.969 7的较高水平之外,与其他7个居群的相似系数均较小。而老山、茅山、青龙山和琅琊山4个居群根中水溶性成分的组成极为相似,居群间的相似系数均在大于0.99的很高水平。聚类结果将10个居群的明党参分成3类。结论:明党参居群间根中水溶性成分存在丰富的多样性,明显地分为3个类型。
Objective: To analyze water-soluble components in the roots of Ch.smyrnioides among different populations that distributed in the main areas and give a reference for germplasm evaluation and quality control.Method: Water-soluble components were extracted with the cold-soaking method and analyzed by HPLC,similarity coefficient was calculated by included angle cosine method according to relative content of major water-soluble components,and systematic relationships were constructed based on UPGMA method.Result: There was significant difference in water-soluble components in root among population.Jiuhuashan population had the highest content of water-soluble extract.The content of water-soluble extract was below the pharmacopoeia standard in the root of Dalongshan population and Fushan population.There was significant difference in the HPLC chromatogram of water-soluble components in the root of Ch.smyrnioides from different populations,and the number of common peak was small.Similarity coefficient significantly ranged from 0.030 6 to 0.999 5 among 10 populations of Ch.smyrnioides.Water-soluble components in the root of Zijinshan population was the most unique,similarity coefficients were relatively small among Zijinshan population and the other seven populations except Hongshan population,and similarity coefficient was in a higher level of 0.969 7 between Zijinshan population and Hongshan population.Water-soluble components were extremely similar in four populations that were Laoshan,Maoshan,Qinglongshan and Langyashan,and similarity coefficients among them were in a high level exceeded 0.99.10 populations were divided into 3 groups according to clustering results.Conclusion: Water-soluble components show a high diversity in the roots of Ch.smyrnioides among different populations,and can be clearly divided into 3 types.

关键词(KeyWords): 明党参;种质资源评价;水溶性成分;HPLC;质量控制
Changium smyrnioides;germplasm evaluation;water-soluble components;HPLC;quality control

Abstract:

Keywords:

基金项目(Foundation): 国家科技攻关计划项目(2001BA701A59,2001BA701A62-04);; 国家科技重大专项(2009ZX09308-002)

作者(Author): 王长林;郭巧生;程搏幸;杨丽雯;周廷惠;
WANG Changlin,GUO Qiaosheng,CHENG Boxing,YANG Liwen,ZHOU Tinghui(Institute of Chinese Medicinal Materials,Nanjing Agricultural University,Nanjing 210095,China)

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