赵玉洋;杨健;查良平;谢冬梅;黄璐琦;袁媛;
ZHAO Yu-yang;YANG Jian;ZHA Liang-ping;XIE Dong-mei;HUANG Lu-qi;YUAN Yuan;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

• 1:中国中医科学院中药资源中心道地药材国家重点实验室培育基地

摘要(Abstract)：

该文将双分子标记法用于厚朴与凹叶厚朴木质素类成分的评价中,首先通过比较厚朴和凹叶厚朴化学成分,发现二者叶片中magnolignanⅠ的含量存在显著差异;然后进行厚朴与凹叶厚朴转录组测序,并选择与厚朴木质素类成分结构最相似的p-hydroxyphenyl lignin,进行生物合成关键酶基因挖掘。通过比较厚朴木质素类成分相关同源基因在厚朴和凹叶厚朴中的表达水平和结构变异,筛选获得1个编码肉桂醇脱氢酶基因片段作为厚朴木质素类成分双分子评价的候选标记,为进一步建立厚朴优劣双分子评价方法提供技术支撑。
The study used use bimolecular marking methods to evaluate the lignans of Magnolia officinalis and M. officinalis var.biloba. First,we compare the chemical constituents between M. officinalis and M. officinalis var. biloba. There were significant differences in concentration of magnolignan I between leaves of these two varieties. Then we further select the p-hydroxyphenyl lignin to mining the key enzyme genes of biosynthesis from Magnolia transcriptome,and screened an encoding cinnamyl alcohol dehydrogease gene as the candidate marker of bimolecular marking methods of Magnolia quality by comparing of the expression level and structure variation in homologous gene between M. officinalis and M. officinalis var. biloba. The established method provides the technical support for bimolecular marking methods of Magnolia quality evaluation.

关键词(KeyWords)： 厚朴;木质素;双分子评价;肉桂醇脱氢酶
Magnolia officinalis;lignins;bimolecular marking methods;cinnamyl alcohol dehydrogease

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划(863)项目(SS2014AA022201);; 国家中医药管理局中医药行业科研专项(201407003)

作者(Author): 赵玉洋;杨健;查良平;谢冬梅;黄璐琦;袁媛;
ZHAO Yu-yang;YANG Jian;ZHA Liang-ping;XIE Dong-mei;HUANG Lu-qi;YUAN Yuan;State Key Laboratory of Dao-di Herbs Breeding Base,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

Email:

DOI:

参考文献(References)：

• [1]王尧龙,黄璐琦,袁媛,等.药用植物转录组研究进展[J].中国中药杂志,2015,40(11):2055.
• [2]黄璐琦,钱丹,邓超.双分子标记法的构建及在中药研究中的应用[J].中国中药杂志,2015,40(2):165.
• [3]杨占南,孙一铭,罗世琼.厚朴和凹叶厚朴的质量评价[J].广东农业科学,2012(15):108.
• [4]彭博,贺蓉,杨滨,等.厚朴和凹叶厚朴对实验性胃肠动力障碍的药效作用差异研究[J].中国中药杂志,2010,35(19):2624.
• [5]袁媛,于军,黄璐琦,等.基于同源基因功能分化的药用植物活性成分变异机制研究[J].中国中药杂志,2015,40(6):1023.
• [6]吴凯朝,黄诚梅,李杨瑞,等.Trizol试剂法快速高效提取3种作物不同组织总RNA[J].南方农业学报,2012,43(12):1934.
• [7]卢戌.基于第二代测序转录组组装软件比较研究[D].兰州:兰州大学,2013.
• [8]Li B,Dewey C N.RSEM:accurate transcript quantification from RNA-Seq data with or without a reference genome[J].BMC Bioinformatics,2011,12(1):323.
• [9]Brastianos P K,Horowitz P M,Santagata S,et al.Genomic sequencing of meningiomas identifies oncogenic SMO and AKT1mutations[J].Nat Genet,2013,45(3):285.
• [10]Drozdetskiy A,Cole C,Procter J,et al.JPred4:a protein secondary structure prediction server[J].Nucleic Acids Res,2015,43:332.