中国中药杂志

2001, (02)

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塞隆骨原动物高原鼢鼠核基因18SrRNA序列测定与分析
Nuclear Ribosomal RNA Small Subunit (18S rRNA) Nucleotide Sequencing and Characterization of Sailonggu(Whole Bone of Myospalax baileyi Thomas)

曹晖,张绍来,周开亚
CAO Hui 1, LIU Yu ping 1, ZHANG Shao lai 2, ZHOU Kai ya 3 (1. Institute of Chinese Materia Medica, China Academy of Traditional Cinese Medicine, Beijing 100700,China; 2. Beijing Tongrentang Medicinal Wine Factory, China Tongrentang Group Comp

摘要(Abstract):

目的 :测定仓鼠科动物高原鼢鼠Myospalaxbaileyi的核rDNA基因序列 ,为塞隆骨正品基原检定提供分子依据。方法 :采用PCR直接测序技术测定高原鼢鼠 18SrRNA基因核苷酸序列并作序列特征分析。结果 :高原鼢鼠的 18SrRNA序列长度为 185 1bp。根据排序比较 ,高原鼢鼠与 2种鼠科动物间的DNA序列同源性为 72 .0 4%~ 72 .18%。结论 :通过基因序列分析 ,DNA测序技术可成为塞隆骨正品基原检定的准确有效手段。
Objective: Sequencing the nuclear ribosomal RNA small subunit (18S rRNA) gene of Myospalax baileyi (Cricetidae) to develop an ultimate and definitive means for origin identification of genuine Sailonggu. Methods: The total DNA was prepared from dried tail tissues. The nuclear 18S rRNA gene region was amplified by PCR using a consensus primer set and its nucleotide sequence was determined by PCR direct sequencing. The characteristic analysis of 18S rRNA sequences was generated usin software program Genetyx SV/R Version 10.1. Results: The entire 18S rRNA gene region of M. baileyi spanned 1851 bp in length. Although multiple alignment of sequence indicates that there are only lower homology (72.04%~72.18%)comparing with its two alias Mus musculus (GenBank Accession number X00686)and Rattus norvegicus (M11188)(Muridae), their highly conservative domain is located in 1020~1509 nt. There are many variable sites from upstream of 5' end, which coud provide a novel information for molecular recognition of Sailonggu. Conclusion:DNA sequencing could be a useful and reliable tool in the origin identification of genuine Sailonggu. [

关键词(KeyWords): 塞隆骨;高原鼢鼠;18SrRNA基因;DNA测序
Sailonggu; Myospalax baileyi ; 18S rRNA gene;DNA sequencing

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基金项目(Foundation): 北京市自然科学基金!资助课题 (7982 0 2 8)

作者(Author): 曹晖,张绍来,周开亚
CAO Hui 1, LIU Yu ping 1, ZHANG Shao lai 2, ZHOU Kai ya 3 (1. Institute of Chinese Materia Medica, China Academy of Traditional Cinese Medicine, Beijing 100700,China; 2. Beijing Tongrentang Medicinal Wine Factory, China Tongrentang Group Comp

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