曹晖,张绍来,周开亚
CAO Hui 1, LIU Yu ping 1, ZHANG Shao lai 2, ZHOU Kai ya 3 (1. Institute of Chinese Materia Medica, China Academy of Traditional Cinese Medicine, Beijing 100700,China; 2. Beijing Tongrentang Medicinal Wine Factory, China Tongrentang Group Comp

• 1:中国中医研究院中药研究所!北京100700
• 2:中国北京同仁堂集团公司北京同仁堂药酒厂!北京101149
• 3:南京师范大学遗传资源研究所!江苏南京210097

摘要(Abstract)：

目的 :测定仓鼠科动物高原鼢鼠Myospalaxbaileyi的核rDNA基因序列 ,为塞隆骨正品基原检定提供分子依据。方法 :采用PCR直接测序技术测定高原鼢鼠 18SrRNA基因核苷酸序列并作序列特征分析。结果 :高原鼢鼠的 18SrRNA序列长度为 185 1bp。根据排序比较 ,高原鼢鼠与 2种鼠科动物间的DNA序列同源性为 72 .0 4%～ 72 .18%。结论 :通过基因序列分析 ,DNA测序技术可成为塞隆骨正品基原检定的准确有效手段。
Objective: Sequencing the nuclear ribosomal RNA small subunit (18S rRNA) gene of Myospalax baileyi (Cricetidae) to develop an ultimate and definitive means for origin identification of genuine Sailonggu. Methods: The total DNA was prepared from dried tail tissues. The nuclear 18S rRNA gene region was amplified by PCR using a consensus primer set and its nucleotide sequence was determined by PCR direct sequencing. The characteristic analysis of 18S rRNA sequences was generated usin software program Genetyx SV/R Version 10.1. Results: The entire 18S rRNA gene region of M. baileyi spanned 1851 bp in length. Although multiple alignment of sequence indicates that there are only lower homology (72.04%～72.18%)comparing with its two alias Mus musculus (GenBank Accession number X00686)and Rattus norvegicus (M11188)(Muridae), their highly conservative domain is located in 1020～1509 nt. There are many variable sites from upstream of 5' end, which coud provide a novel information for molecular recognition of Sailonggu. Conclusion:DNA sequencing could be a useful and reliable tool in the origin identification of genuine Sailonggu. [

关键词(KeyWords)： 塞隆骨;高原鼢鼠;18SrRNA基因;DNA测序
Sailonggu; Myospalax baileyi ; 18S rRNA gene;DNA sequencing

Abstract:

Keywords:

基金项目(Foundation): 北京市自然科学基金!资助课题 (7982 0 2 8)

作者(Author): 曹晖,张绍来,周开亚
CAO Hui 1, LIU Yu ping 1, ZHANG Shao lai 2, ZHOU Kai ya 3 (1. Institute of Chinese Materia Medica, China Academy of Traditional Cinese Medicine, Beijing 100700,China; 2. Beijing Tongrentang Medicinal Wine Factory, China Tongrentang Group Comp

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